| About this Abstract |
| Meeting |
MS&T23: Materials Science & Technology
|
| Symposium
|
3D Printing of Biomaterials and Devices
|
| Presentation Title |
Utilizing Chaotic Advection to Bioprint Hydrogel Sheets with User-Defined, High-Resolution Internal Cell Layers |
| Author(s) |
Ryan Hooper, Cynthia González, Amanee Abu Arish, Anna Beck, Caleb Cummings, Ciro Rodríguez, Grissel Trujillo de Santiago, Mario Moisés Alvarez, David Dean |
| On-Site Speaker (Planned) |
Ryan Hooper |
| Abstract Scope |
For 3D bioprinting to reach clinical viability, it must replicate the microscopic complexities of tissue layers in the body. We are developing a novel, patent-pending bioprinting strategy that utilizes chaotic advection to produce adjacent layers of two different cell-seeded hydrogels within an extruded construct. The number and thickness of these layers can be modulated by the user, achieving resolutions at the scale of individual cells (~10 microns). We are now extruding thin, wide, hydrogel sheets (e.g., with 0.5 x 10 mm oblong cross-sectional dimensions) containing alternating internal layers of two hydrogels for the first time with consistent geometries. We have demonstrated the biocompatibility of Sodium Alginate-Gelatin Methacryloyl (SA-GelMA) hydrogels with human Mesenchymal Stem Cells (hMSCs), as well as the ability to perfuse constructs with vacated internal layers in a bioreactor system. In vitro and in vivo (i.e., murine) modeling is ongoing to validate our strategy in novel tissue engineering applications. |