About this Abstract |
Meeting |
MS&T23: Materials Science & Technology
|
Symposium
|
3D Printing of Biomaterials and Devices
|
Presentation Title |
Bone Tissue Engineering under Fluid Flow Conditions for Development of Invitro Testbeds of Cancer Metastasis |
Author(s) |
Kalpana S. Katti, Dinesh R Katti, Haneesh Jasuja, Quyen Hoang, Hanmant Gaikwad, Shrinwanti Ghosh, Preetham Ravi, Dipayan Sarkar, Kalidas Shetty, Anu Gaba, Parth Vyas, Sharad V Jaswandkar |
On-Site Speaker (Planned) |
Kalpana S. Katti |
Abstract Scope |
An emerging market for bone tissue engineering is therapies for bone metastasis of prostrate and breast cancer and robust in vitro models of metastasis. We have developed novel bone-mimetic testbeds of cancer using tuning of nanoclays with amino acids to create a bone-niche to which cancer cells migrate. Cancer migration and attachment to bone is enabled using specially designed bioreactors that provide physiologically relevant shear forces. Further, the use of patient-derived cells lines of cancer provide a realistic model of bone metastasis. The testbed is evaluated for the effectiveness of specialty crop derived compounds towards metastasis. The cancer cells influence osteogenesis, affecting prognosis for patients due to extensive skeletal failures. The adhesion forces between cancer cells and bone are measured AFM-based techniques. The attachment of cancer cells to bone is experimentally evaluated as well as modeled through activation of integrins. The novel testbed represents next-generation platform for investigation bone metastasis. |