| About this Abstract |
| Meeting |
MS&T23: Materials Science & Technology
|
| Symposium
|
3D Printing of Biomaterials and Devices
|
| Presentation Title |
Impact of Fluid Flow on Bone Metastasis of Prostate Cancer: Invitro Testbeds of Bone Metastasis |
| Author(s) |
Dinesh Katti, Haneesh Jasuja, Quyen Hoang, Preetham Ravi, Parth Vyas, Sharad V Jaswandkar, Kalpana Katti |
| On-Site Speaker (Planned) |
Dinesh Katti |
| Abstract Scope |
Prostate and breast cancer deaths usually occur from the metastasis of cancer to bone. We have created a novel 3D tissue-engineered polymeric scaffold seeded with hMSCs. The bone-mimetic scaffold is further seeded with patient-derived and commercial cell lines of prostate cancer after bone growth to create a testbed of prostate cancer bone metastasis. In order to recapitulate the fluid-derived shear stresses experienced by cancer cells due to the blood flow, we have fabricated a novel bioreactor using 3D printing. The CXCR4 levels are important regulators of the invasiveness of cancer and metastasis, and interestingly, the flow does not alter the CXCR4 levels, but the proximity of bone does. Under fluid flow, responses of cancer cells and interactions with the bone are influenced by integrin activation and upregulation of αvβ3 integrins and MMP-9 levels causing increased migration. These studies describe the important role of interstitial fluid flow in prostate cancer metastasis. |