| About this Abstract |
| Meeting |
2024 TMS Annual Meeting & Exhibition
|
| Symposium
|
Advanced Biomaterials for Biomedical Implants
|
| Presentation Title |
Design and Application of Porous PDMS Membrane Devices in Nude Mice for Localized Treatment of Triple Negative Breast Cancer |
| Author(s) |
Yiporo Danyuo, Azeko Ali Salifu, John David Obayemi, Stanley Eluu, Toyin Aina, Josephine Oparah, Precious Etinaso , Fred McBagonluri , Wole W. Soboyejo |
| On-Site Speaker (Planned) |
Yiporo Danyuo |
| Abstract Scope |
This paper presents an in vitro and in vivo study of porous PDMS-based membrane devices (P-PDMS-MD) obtained via solvent casting. Cancer drugs (prodigiosin and paclitaxelTM) were conjugated to Luteinizing-Hormone-Releasing-Hormone (LHRH) at concentrations of 0.5 mg/ml for specific targeting. Drug releases were investigated on the cytotoxic effects on cultured cells (MBA-MD-231 and MCF10A) using AlamarBlue assay. Induction of apoptosis was examined by flow cytometry following cell staining. In vivo study was done using Athymic Nude-Foxn1nu strain mice following an approved protocol. The tumor tissues and tissues from major organs (kidneys, liver, and lungs) were harvested for cytotoxic studies and histopathological analysis. The results demonstrated no tumour regrowth in the treated groups with strong inhibitory effects of the conjugated drugs on tumor regrowth. The histological images of liver, lungs, and kidney, an indication of no toxicity or detrimental effects. Results were discussed for the use of -PDMS-MD for localized cancer treatment. |
| Proceedings Inclusion? |
Planned: |
| Keywords |
Biomaterials, Other, Other |