| About this Abstract |
| Meeting |
MS&T21: Materials Science & Technology
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| Symposium
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Next Generation Biomaterials
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| Presentation Title |
In Vivo And In Vitro Evaluation of PLGA-PEG Microspheres Loaded with LHRH-Targeted Drugs for Effective Breast Cancer Treatment.
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| Author(s) |
Sandra Musu Jusu, John David Obayemi, Ali Azekoo Salifu, Chukwudalu Clare Nwazojie, Vanessa Uzonwanne, Olushola S. Odusanya, Winston Oluwole Soboyejo |
| On-Site Speaker (Planned) |
Sandra Musu Jusu |
| Abstract Scope |
This study focused on developing targeted drug systems to treat and prevent locally recurring TNBC tumors after surgical resection. In the study, we developed a blend of FDA-approved polylactic-co-glycolic acid-polyethylene glycol polymeric microspheres encapsulating targeted therapeutic agents. We functionalized these therapeutic agents with LHRH-Releasing hormone ligands. We prepared PLGA-PEG microspheres using solvent evaporation technique, characterizing them in terms of their physicochemical, structural, morphological, and thermal properties. The in vitro drug release was done at human body temperature and hyperthermic temperatures. Transient concentrations were measured using UV visible spectrophotometer. Kinetic and thermodynamic models were used to characterize the in vitro drug release from PLGA-PEG microspheres. This revealed a non-Fickian drug release, well-characterized by the Korsmeyer-Peppas model and thermodynamically non-spontaneous. Under in vitro and in vivo conditions, LHRH-conjugated drugs greatly reduced TNBC’s cell/tissue viability and prevented its loco-regional recurrence. Kaplan–Meier analysis revealed significantly extended survival for treated mice post-surgical tumor resection. |