About this Abstract |
Meeting |
MS&T21: Materials Science & Technology
|
Symposium
|
Next Generation Biomaterials
|
Presentation Title |
Enzymes Immobilized on Nanocarriers for the Degradation of Synthetic Polymers |
Author(s) |
Eva Krakor, Sanjay Mathur, Isabel Gessner, Michael Wilhelm |
On-Site Speaker (Planned) |
Eva Krakor |
Abstract Scope |
Sustainable concepts for the degradation of omnipresent industrial plastics are a pressing environmental and societal need. Therefore enzymes (lipase and cutinase) were covalently immobilized on carrier nanoparticles (SiO2 and Fe3O4@SiO2) through 3-(aminopropyl)trimethoxysilane (APTMS) linkers that provided terminal amino groups for forming a stable bond to enzyme molecules upon addition of glutaraldehyde. The presence of enzymes on the surface was confirmed by zeta potential and XPS measurements, while their degradation activity and long-term stability over a time-period of 144 hours was demonstrated by monitoring the conversion of 4-nitrophenyl acetate (4-NPA) to 4-nitrophenol (4-NP) by UV-Vis spectroscopy. Additionally, enzymatic decomposition of PCL was verified through SEM and mass loss analyses of intermediate stages. |