| Abstract Scope |
Cardiovascular disease is a global leading cause of death, necessitating over a million bypass and vessel replacement surgeries annually. Many patients lack suitable donor tissue due to other health issues or insufficient tissue from previous surgeries. Tissue engineering offers a promising alternative to generate viable vascular grafts. This study aims to develop a chitosan (CS) and gelatin (Gel) cellular scaffold reinforced with graphene oxide (GO), synthesized via electrospinning, to support endothelial cell culture for vascular grafts. Methods such as FTIR, UV spectroscopy, mechanical testing, SEM, and MTT determined the scaffolds' physicochemical properties. The GO-reinforced nanofibers exhibited enhanced diameter, porosity, and mechanical strength, leading to higher cell viability and proliferation after 24 and 48 hours. GO-reinforced CS and Gel nanofibers offer a platform for vascular tissue generation. |