|About this Abstract
||2022 TMS Annual Meeting & Exhibition
||BioNano Interfaces and Engineering Applications
||K-15: Potentiometric Immunosensor for the Selective Thyroxine Detection Using Cu-MOF@PANI Composite in Serum Samples
||Mradula Mradula, Rocky Raj, Sunita Mishra
|On-Site Speaker (Planned)
A label-free electrochemical immunosensor was fabricated for detection of thyroxine hormone by immobilizing anti-thyroxine monoclonal antibody (Ab) onto copper metal-oxide framework (Cu-MOF) and polyaniline (PANI) composite modified screen-printed carbon electrode (SPCE). Cu-MOF and their composite were characterized by field emission scanning electron microscopy (FESEM), Fourier transform infrared spectroscopy (FTIR), RAMAN spectroscopy, X-ray diffraction (XRD), and cyclic voltammetry (CV). The immunosensor possessed a dynamic linear range (10–105 pM) with a low limit of detection (LOD) of 0.33 pM (0.25 pg/ml) for CV. The evaluation study of immunosensor displayed a good analytical recovery of ≥ 90% when the different concentration of thyroxine was added in serum sample. In addition, to determine the reproducibility for working electrode between and within batch, the relative standard deviation calculated were 3.87% and 6.04%, respectively, represent it's good reproducibility. The immunosensor retained 90% of its initial activity after its regular use for 40 days, while being stored at 4 °C.
||Nanotechnology, Biomaterials, Other