|About this Abstract
||2022 TMS Annual Meeting & Exhibition
||BioNano Interfaces and Engineering Applications
||<i>In vitro</i> Pre-osteoblast Cell Differentiation by Luminescent Hydroxyapatite
||Fabian Martinez, Karla Juárez-Moreno, Manuel Herrera, Olivia A. Graeve
|On-Site Speaker (Planned)
We present a differentiation study of murine pre-osteoblast cells in the presence of hydroxyapatite undoped and doped with rare-earth elements, including Eu<sup>2+</sup>, Eu<sup>3+</sup>, Ce<sup>3+</sup>, Tb<sup>3+</sup>, and Yb<sup>2+</sup>. Before assay studies, the hydroxyapatite powders were analyzed by X-ray diffraction and spectral cathodoluminescence, confirming that the dopants are present in the powders in amounts of a few atomic percent and they do not modify the equilibrium phase of hydroxyapatite. Alkaline phosphatase activity assay on the surface of treated cells, demonstrates that the incorporation of rare earth elements in the structure of hydroxyapatite modifies its effect upon cell differentiation. Moreover, to unravel whether the rare-earth elements drive an osteoconductive response, ongoing experiments are being completed to quantify the amount of differentiation gene biomarkers. We found that the incorporation of the species Eu<sup>2+</sup>, Eu<sup>3+</sup>, and Yb<sup>2+</sup>, into the crystal structure of the hydroxyapatite enhances significantly the differentiation of the pre-osteoblast cells.
||Biomaterials, Ceramics, Powder Materials