|About this Abstract
||2022 TMS Annual Meeting & Exhibition
||BioNano Interfaces and Engineering Applications
||Mechanics of Cellular Actin Filaments
||Dinesh Katti, Sharad V Jaswandkar, Kalpana S. Katti
|On-Site Speaker (Planned)
In recent years, significant research efforts are being made to link cell mechanics and human diseases. In eukaryotic cells, the cytoskeleton is a three-dimensional dynamic structure that helps cells retain their form, internal organization, and mechanical rigidity. The cytoskeleton comprises the actin microfilaments, microtubules, and intermediate filaments. Our prior work has shown that the cell mechanics are significantly altered during cancer progression. We have also shown that the cancer cells' altered mechanical properties during disease progression are linked to the actin reorganization. In the current study, steered molecular dynamics (SMD) simulations have been used to study the actin mechanics. We find that the conformational locks and nonbonded interaction at interstrand and intrastrand interfaces regulate the F-actin dynamics. Also, the ADF/cofilin-bound F-actin molecular model elucidates the F-actin severing and depolymerization mechanisms. The F-actin mechanics described here are essential for constructing a mechanobiological eukaryotic cell model with disease progression.
||Biomaterials, Mechanical Properties, Modeling and Simulation