|About this Abstract
||MS&T22: Materials Science & Technology
||3D Printing of Biomaterials and Devices
||The Regulatory Roles of the Substrate Microenvironment in Cancer Progression in Tissue Engineering Scaffolds
||Dinesh Katti, Sharad V Jaswandkar, Hanmant Gaikwad, Kalpana S. Katti
|On-Site Speaker (Planned)
The substrate microenvironment and mechanical cues influence cancer progression in in vitro systems. The presence of extracellular minerals modulates membrane proteins' conformation (such as integrin), resulting in signal transduction within the cells. Thus, cancer cell mechanobiology, influenced by cell-microenvironment interaction such as substrate stiffness and biochemical/topographic substrate properties, plays a vital role in cancer cell growth and metastasis. Our present AFM-based experimental study and finite element analysis-based computation study focuses on understanding the influence of physical features of the cellular environment, such as bonemimetic scaffold substrate stiffness, on the cancer cell's metastatic characteristics like cell adhesion. Our investigations on the cellular adhesion protein integrin (αVβ3) and the cellular cytoskeletal protein actin provide insight into their mechanical response, critical to cellular functions. Furthermore, the studies on integrin interaction with the nanoclay provide an insight into stem cell differentiation leading to bone tissue regeneration in scaffolds.