|About this Abstract
||MS&T23: Materials Science & Technology
||Society for Biomaterials: Biological Response to Materials and Material’s Response to Biological Environments
||Cancer Cell-substrate Microenvironment Crosstalk in Metastasis Progression in Tissue Engineering Scaffolds
||Dinesh Katti, Sharad V Jaswandkar, Hanmant Gaikwad, Kalpana Katti
|On-Site Speaker (Planned)
The bone microenvironment and mechanical cues like fluid flow influence metastasis. The presence of extracellular minerals modulates membrane proteins' conformation, resulting in signal transduction within the cells. We report extensive computational studies in tandem with experiments on invitro testbeds of cancer metastasis to the bone using patient-derived cell lines of breast and prostate cancer in bioreactors. We evaluate the mechanics of both internal moieties (actins) as well as external (integrins) that influence the mechanics of cells. Actin filaments polymerization and depolymerization are key to the cell-cell adhesion observed in tumor formation. Our modeling studies elucidate detailed mechanisms of the cofilin decorations that influence the polymerization and depolymerization of the actins on loading. Our investigations on the cellular adhesion protein αvβ3 integrin and the cytoskeletal protein actin provide insight into their mechanical response, critical to cellular function, and provide an insight into stem cell differentiation leading to bone tissue regeneration.