|About this Abstract
||MS&T23: Materials Science & Technology
||Society for Biomaterials: Biological Response to Materials and Material’s Response to Biological Environments
||Topographical Effect on Hs27 Fibroblast Response
||Chunghwan Kim, Michael Robitaille, Joseph A. Christodoulides, Yisha Ng, Marc Raphael, Wonmo Kang
|On-Site Speaker (Planned)
Contact guidance is known as how cells are guided by an external topography. The morphological and dynamic cell responses are strongly influenced by different topographical features such as lateral and vertical dimensions, namely, ridge and groove widths and groove depth. However, experimental studies that independently quantify the effect of the individual dimensions as well as their coupling on cellular function are still limited particularly under the subcellular scales. In this work, we perform extensive parametric studies in the dimensional space–well beyond the previously explored range in the literature–to explore topographical effects on the cellular morphology and migration through static and dynamic analyses of live cell images. Our static and dynamic analyses reveal that the groove depth is statistically the most important feature, influencing the cell elongation, alignment as well as directional migration. Possible mechanisms have been considered to offer mechanistic explanations of the observed cell behaviors.