|About this Abstract
||MS&T23: Materials Science & Technology
||Society for Biomaterials: Biological Response to Materials and Material’s Response to Biological Environments
||Surface Modification Strategies for Inhibiting Biofilm Formation on Biomaterials
||Christopher Siedlecki, Alyssa Ochetto, Chen Chen, Dongxiao Sun, Asma Khursheed, Harry Allcock, Lichong Xu
|On-Site Speaker (Planned)
Biomaterial centered infections remain an impediment to the use of implantable medical devices. These infections arise from initial adhesion of bacteria to a surface, followed by proliferation and maturation into biofilms. Mature biofilms exude an extracellular covering referred to as extracellular polymeric substances (EPS) which protects the bacterial community from antibiotics as well as normal immune responses. We have developed methods to affect both initial attachment of bacteria as well as the subsequent maturation. In recent work, we identified small molecules that interfere with intracellular signaling molecules in bacteria. Using nitric oxide and small molecules derivates of 4-arylazo-3,5-diamino-1H-pyrazole as inhibitors of intracellular nucleotide signaling molecules, biofilms were largely eradicated from the surface with orders of magnitude reductions in viable bacteria. Further, we show that these molecules and disruption of the biofilm allows for increased efficacy of antibiotics, with 2 ug/ml ciprofloxacin generating 3 orders of magnitude reductions in viable bacteria.