Electrospun chitosan membranes have been proven to be beneficial in wound applications due to their ability to release therapeutics over time. In this study, we use an alternative soaking method to load trans-2-decenoic acid (T2DA) on membranes treated with decanoic and hexanoic fatty acid chains to observe the release kinetics. This method is advantageous over previous techniques due to its ability for uniform absorption in larger samples. Forty membranes, similar in mass and size, were loaded with 7ml of ethanol combined with either 40, 80, or 120mg of T2DA. The controlled environment of the solutions mimics the aqueous environment conditions of body fluids to predict human response. By attaching these chains, we increase the hydrophobicity of the membranes and prevent swelling in aqueous conditions. Preliminary results show that decanoic-modified membranes were more absorbent than hexanoic-modified membranes. Similarly, those loaded with higher concentrations of T2DA released more than lower concentrations.