Abstract Scope |
In 3D bioprinting, the bioink is precisely deposited on the substrate to build 3D cellular constructs based on a layer-by-layer mechanism. Photocrosslinkable polymers such as gelatin methacrylate (GelMA) have been widely utilized in various 3D bioprinting applications. When the GelMA is used for 3D bioprinting, the photoinitiator is required for its crosslinking. Two types of photoinitiator are commonly used: Irgacure 2959 and lithium phenyl-,2,4,6-trimethylbenzoylphosphinate (LAP). This study compares effects of these two photoinitiators on the biomechanical properties of GelMA hydrogels and cell viability in 3D bioprinting process. The biomechanical properties include swelling, degradation, and stress-strain relationship. This study also compares the effects of these two photoinitiators on the cell viability during and after 3D bioprinting process. Specifically, during the 3D bioprinting process the effects of the photoinitiator concentration and printing time are investigated, and after the 3D bioprinting process the effects of the printing time and incubation time are investigated. |