|About this Abstract
||Materials Science & Technology 2020
||Next Generation Biomaterials
||Nanostructured Surface Bioactive Composite Scaffold for Filling of Mandibular Bone Defects: A Pilot Study
||Leonardo Ciocca, Claudio Marchetti, Achille Tarsitano
|On-Site Speaker (Planned)
Purpose: The aim of this study was to investigate the chemical and physical surface properties of a hybrid nano-hydroxyapatite/collagen/polycaprolactone (nHA/Coll/PCL) material, and to test its in vitro biocompatibility and in vivo osseointegration.
Methods: The material was characterized and the biocompatibility was tested using human mesenchymal stem cells, hepatocyte carcinoma and primary osteogenic sarcoma cells. In addition, the ability of this material to fill three-wall bony defects was tested in the mandible of a sheep, simulating a maxillofacial defect after ablative cancer surgery.
Results: The material confirmed a good homogeneity of the composite HA-coll-PCL, with mineralized fibers of collagen with nano HA. Human bone-marrow mesenchymal stem cells on the surface scaffold showed high metabolic activity and a high rate of viability. Biocompatibility complementary testing demonstrated no significant cytotoxicity. Histological analysis of the in-vivo experimentation showed osteointegration of the material and the absence of inflammatory cells at the bone–scaffold interface.
||Planned: Other (journal publication, etc.; describe below)