| About this Abstract |
| Meeting |
MS&T22: Materials Science & Technology
|
| Symposium
|
3D Printing of Biomaterials and Devices
|
| Presentation Title |
Selective Artificial Neural Network by Targeted Delivery of Neuronal Cells Using Magnetically Controlled 3D Printed Microrobots |
| Author(s) |
Hongsoo Choi |
| On-Site Speaker (Planned) |
Hongsoo Choi |
| Abstract Scope |
Several in vitro neural network models have been developed to mimic the reconstruction and interconnection of neural networks and to study brain function and related diseases. Here we report a 3D magnetically actuated microrobot fabricated by 3D laser lithography based on two-photon polymerization for selective neurite alignment and neuronal connections. Using confocal immunofluorescence imaging, the aligned neurite outgrowth and synaptic connections in the neural network were measured. The microrobot, in which the rat's primary hippocampal cells were cultivated, was transmitted between two neural clusters by a magnetic field and then structurally and functionally connected to a neural network that can transmit neural activity signals. Neuronal activities were measured with a MEA system to monitor the propagation of extracellular axonal signals from the neural clusters. The proposed microrobot shows the potential for in vitro neural experiments to understand how neurons communicate in the neural network by actively connecting neural clusters. |