| About this Abstract |
| Meeting |
2022 TMS Annual Meeting & Exhibition
|
| Symposium
|
2022 Technical Division Student Poster Contest
|
| Presentation Title |
SPG-10: Assessing the Bio-stability of miRNA Conjugated Metal and Metal Oxide Nanoparticles via Electroanalysis |
| Author(s) |
Chaimae El Ghzaoui, Craig J. Neal, Elayaraja Kolanthai, Yifei Fu, Udit Kumar, Carlos Zgheib, Kenneth W. Liechty, Sudipta Seal |
| On-Site Speaker (Planned) |
Chaimae El Ghzaoui |
| Abstract Scope |
Use of non-coding DNA/RNA as gene therapy has allowed treatment of serious, widely varying medical conditions by producing high-specificity bio-responses. To improve therapeutic efficacy, much research has been devoted to use of nanoparticle vehicles decorated with DNA/RNA sequences at their surfaces: increasing bio-molecule stability and/or allowing added therapeutic functionalities (e.g. site-targeting, local reactive oxygen species production (ROS)). In the presented study, we compare specific bio-active characters/properties of three candidate nano-carriers: gold (bioactive, metal), silica (bioinert, oxide) , and cerium oxide (bioactive, oxide) following modification with miRNA 146a. Electroanalytical methods and assays of enzyme-mimetic activities are used to characterize conjugates’ chemical stabilities and radical scavenging activities, in presence of ROS (approximating pathology-associated biochemical environment). Results from these studies suggest a potential synergistic effect (unique ROS scavenging behavior; conjugation-conferred electrochemical stability) from miRNA conjugation to cerium oxide. Similar gold or silica nanoparticle conjugates showed inactivity towards ROS and degradation over cycling, respectively. |
| Proceedings Inclusion? |
Undecided |
| Keywords |
Nanotechnology, Biomaterials, Characterization |