| About this Abstract |
| Meeting |
2021 TMS Annual Meeting & Exhibition
|
| Symposium
|
Bio-Nano Interfaces and Engineering Applications
|
| Presentation Title |
Control of Scaffold Shear Forces Through a Perfusion Bioreactor for Design of Prostate Cancer Bone Metastasis Testbed |
| Author(s) |
Haneesh Jasuja, Akerkouch Lahcen, Trung Le, Dinesh R Katti, Kalpana S. Katti |
| On-Site Speaker (Planned) |
Haneesh Jasuja |
| Abstract Scope |
Prostate cancer (PCa) has a propensity to metastasize to bone, resulting in skeletal impairment and high mortality. Interstitial fluid flow induced mechanical cues are well known for their bone remodeling and cancer promigratory roles. However, the influence of mechanical signals PCa progression at bone metastasis is poorly explored. We developed a 3D in-vitro dynamic model to examine role of fluid derived shear stress on PCa progression to bone. We created a bone niche employing nanoclay based scaffolds and seeded human PCa cells on the tissue-engineered bone in a specially designed bioreactor to recapitulate PCa bone metastasis. Computational fluid dynamics studies suggest a high correlation between fluid velocity magnitude and scaffold pore size. The experimental results indicate uniform and directional distribution of hMSCs and varying PCa tumor morphology on dynamically cultured scaffolds. Thus, shear forces exerted by fluid flow can be used to influence metastasis characteristics of PCa. |
| Proceedings Inclusion? |
Planned: |
| Keywords |
Biomaterials, Composites, Mechanical Properties |