| About this Abstract |
| Meeting |
2021 TMS Annual Meeting & Exhibition
|
| Symposium
|
Bio-Nano Interfaces and Engineering Applications
|
| Presentation Title |
Enhancing Electrochemical Detection of Choline Using Molecularly Imprinted Polymer Electrode |
| Author(s) |
Sermin F. Utku, Sevgul Melahat Bakay, Adil Denizli, Inci Fatma Cilesiz |
| On-Site Speaker (Planned) |
Sevgul Melahat Bakay |
| Abstract Scope |
Molecularly imprinted polymeric surfaces enable quantitative and/or qualitative analysis of analytes using the biological recognition sites created on the electrodes. A molecularly imprinted choline pencil graphite small-molecule biosensor with surfaces containing 0.2 M and 0.5 M choline were generated for the quantitative detection of choline using cyclic voltammetry, electrochemical impedance spectroscopy in a three-electrode electrochemical cell in pM-μM choline solutions. The amount of choline bound to the complementary recognition sites on the electrodes correlated with the change in current, voltage and impedance. TRIS (hydroxymethyl) aminomethane as a competitive small-molecule demonstrated specificity for choline. Blood serum choline concentration was determined as 10.64 ± 0.546 µM. The detection range for the 0.2M electrodes was determined as 71.6 pM-7.16 µM with a 71.6 pM limit. Based on these results, a lower limit of detection of small-molecules has been obtained than those obtained by electrochemical methods only. |
| Proceedings Inclusion? |
Planned: |
| Keywords |
Biomaterials, Surface Modification and Coatings, Nanotechnology |