| About this Abstract |
| Meeting |
MS&T22: Materials Science & Technology
|
| Symposium
|
Society for Biomaterials: Student Poster Contest + Rapid Fire
|
| Presentation Title |
C-16: Decanoic Anhydride-modified Chitosan Membranes Loaded with Bupivacaine and cis-2-decanoic Acid Affect Cytokine Expression of Keratinocytes |
| Author(s) |
Emily Coleman Montgomery, Zoe Harrison, J. Amber Jennings |
| On-Site Speaker (Planned) |
Emily Coleman Montgomery |
| Abstract Scope |
Biomaterials that support the natural inflammatory response and inhibit infections can aid in decreasing the severity of inflammation associated with burn wounds. Electrospun chitosan membranes are promising local drug delivery systems due to their biocompatibility and fibrous nanostructure. Local delivery of cis-2-decenoic acid (C2DA) for infection prevention in combination with an antimicrobial local anesthetic such as bupivacaine (BUP) can address both infection and pain. Chitosan membranes were electrospun using 71% DDA chitosan in 70:30% (v/v) trifluoroacetic acid to dichloromethane. Membrane discs 1 cm in diameter were treated in 50:50 pyridine to decanoic anhydride (DA). DA-modified membranes were loaded with BUP, C2DA, or a combination at low, medium, and high concentrations. Membranes were UV sterilized and directly exposed to normal human epidermal keratinocytes (NHEK) for 24 and 72 hours, and cytokines IL-12 and IL-10 in supernatants were measured using ELISA. Results indicate that C2DA combined with BUP promotes anti-inflammatory cytokine release. |