|About this Abstract
||2022 TMS Annual Meeting & Exhibition
||2022 Technical Division Student Poster Contest
||SPG-12: Dual miRNAs Functionalized Cerium Oxide for Angiogenesis and Anti-inflammatory
||Elayaraja Kolanthai, Aadith Menon, Balaashwin Babu, Yifei Fu, Udit Kumar, Craig Neal, Kenneth W. Liechty, Sudipta Seal
|On-Site Speaker (Planned)
Improper wound healing is a global medical problem with very few viable treatments. The culprit is often the dysregulation in inflammation and angiogenesis of wound areas which ultimately affect wound healing time. This is especially true in diabetic wounds, where a high level of reactive oxidative species (ROS) and low oxygen levels dominate the infected site. We have decorated anti-inflammatory and angiogenesis regulating miRNAs (miRNA-146a and miRNA 23a) on to cerium oxide nanoparticles (CNPs) using bioconjugation methods. The CNP works as a nanocarrier for the miRNA. Conjugation of both miRNAs on CNPs was confirmed by UV-vis, and fluorescence spectroscope/microscope. The particles size of CNPs increased after miRNA bioconjugation. These samples were also tested for biocompatibility, cell transfection efficiency, angiogenesis, and inflammation. Based on these results, the dual miRNA decorated CNPs could control inflammation, increase the amount of oxygen by forming new vascularization, and remove ROS at the wound site.