About this Abstract |
Meeting |
2021 Annual International Solid Freeform Fabrication Symposium (SFF Symp 2021)
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Symposium
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Applications
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Presentation Title |
3D Bioprinting of Cell-laden Scaffolds for Skin Substitutes |
Author(s) |
Fateme Fayyazbakhsh, Michelle Amato, Michael Khayat, Delbert E. Day, Yue-Wern Huang, Ming C. Leu |
On-Site Speaker (Planned) |
Fateme Fayyazbakhsh |
Abstract Scope |
Third-degree burns are hard-to-heal wounds and skin grafts as the gold standard for burn treatment are limited by shortage in donor site, graft rejection, and disease transmittance. In this research, we fabricated cell-laden scaffolds with human primary fibroblasts, gelatin, and alginate. We adopted the extrusion-based 3D printing technology as a minimally-invasive method for bioprinting. Different mixtures of hydrogels and cells are printed and cultured in complete culture media, maintained in a 5% CO2 incubator at 37C for up to 14 days. To evaluate the effect of 3D printing process and bioink composition on cell viability, Live/Dead assay is used, followed by confocal microscopy. The structural integrity of scaffolds is investigated by measuring the rheological behavior, tensile strength, and shape fidelity. The results showed that the scaffolds support 3D cell culture for up to 14 days, which indicates the high potential of the bioprinted scaffolds for third-degree burn treatment. |
Proceedings Inclusion? |
Definite: Post-meeting proceedings |