| About this Abstract |
| Meeting |
2024 TMS Annual Meeting & Exhibition
|
| Symposium
|
Biological Materials Science
|
| Presentation Title |
Dynamic Stress-induced Osteoblast Attachment on 3D Nanoarchitected Scaffold-on-Chip |
| Author(s) |
Alessandro Maggi, Michael Abrams, Kailin Chen, Alexander Bolaņos-Campos, Julia R Greer, Ottman Tertuliano |
| On-Site Speaker (Planned) |
Ottman Tertuliano |
| Abstract Scope |
The underlying mechanisms leading to bone loss and fracture risk are not fully understood at the cellular level. Decades of studies on compliant 2D surfaces and 3D hydrogels show mechanical stimulation of the bone ECM triggers osteogenesis. How dynamic mechanical stimulation of the ECM transfers deformations to cells in 3D and the cell response remains difficult quantify. Here, we fabricate and dynamically compress periodic, nano-architected scaffolds seeded with SAOS osteoblast like cells. Experimentally increasing frequency of dynamic loading up to 3 Hz results an increase in adhesion and f-actin, yet independently changing applied stress or strain applied to the scaffolds did not produce measurable differences in these cell responses. Theory and finite element calculations showed that fluid shear stresses increase with frequency and develop a spatial gradient within a scaffold. The platform enables decoupling aspects of 3D cell-matrix interactions by controlling time-dependent solid and fluid stresses in an engineered ECM. |
| Proceedings Inclusion? |
Planned: |
| Keywords |
Biomaterials, Additive Manufacturing, Mechanical Properties |