|About this Abstract
||2023 TMS Annual Meeting & Exhibition
||Late News Poster Session
||D-39: Platinized Carbon Nanoelectrodes for Electrochemical Measurement of Reactive Oxygen and Nitrogen Species in Neural Stem Cells
||Donghoon Lim, Hyun S. Ahn
|On-Site Speaker (Planned)
It is important to analyze how reactive oxygen and nitrogen species (ROS/RNS) in stem cells affect the differentiation process. To accomplish this, Nanoelectrodes that are small enough not to damage single cells are essential. In this work, we fabricated and characterized platinized carbon nanoelectrodes by pyrolysis of butane inside a quartz capillary and used them as scanning electrochemical microscopy (SECM) tips. Using this nanoelectrode, it was possible to measure the four major ROS/RNS (i.e., H2O2, ONOO-, NO•, and NO2-) in the cytoplasm of neural stem cells (NSCs). The four primary ROS/RNS in single NSCs was monitored using a four-step chronoamperometric method.
||Energy Conversion and Storage, Nanotechnology, Other