About this Abstract |
Meeting |
2022 TMS Annual Meeting & Exhibition
|
Symposium
|
Advances in Powder and Ceramic Materials Science
|
Presentation Title |
Immobilization of Laccase on CuO Nanoparticles: Stability, Catalytic Activity, and Cu Ion Exchange |
Author(s) |
F. Javier Suarez, Rafael Vazquez-Duhalt, Olivia A. Graeve |
On-Site Speaker (Planned) |
F. Javier Suarez |
Abstract Scope |
Immobilization of enzymes is widely used to increase the stability and reusability of catalysts. Laccases have shown an increased stability and catalytic activity when immobilized in copper oxide nanoparticles (CuO-NPs). However, the role of the CuO-NPs in the immobilization of laccases remains unclear. It has been hypothesized that the increase in both stability and catalytic activity is caused by the exchange of Cu ions between the laccases and the CuO-NPs. In this study we immobilized laccase from <i>Coriolopsis gallica</i> on CuO-NPs by covalent attachment using (3-aminopropyl) triethoxysilane (APTES) as linking molecule. The results demonstrated an extraordinary increase in stability and catalytic activity of laccase, showing higher kcat and total turnover number (TTN). Inductively coupled plasma mass spectrometry was used to determine the exchange of Cu ions between the laccase and the CuO-NPs, resulting in an improved understand of the laccase-CuO interface. |
Proceedings Inclusion? |
Planned: |
Keywords |
Ceramics, Nanotechnology, Powder Materials |