| About this Abstract |
| Meeting |
MS&T22: Materials Science & Technology
|
| Symposium
|
Society for Biomaterials: Biological Response to Materials and Material’s Response to Biological Environments
|
| Presentation Title |
Wear Particle-induced Differentially Polarized Macrophages Exhibit Variation in Protein Profile than M1 and M2 |
| Author(s) |
Divya Bijukumar, Vaishnavi Beena Valsan, Ryan Bomgarden, Guoxing Zheng, Mark Barba, Deborah Hall, Mathew Thoppil Mathew, Robin Pourzal |
| On-Site Speaker (Planned) |
Divya Bijukumar |
| Abstract Scope |
Biological factors from the local microenvironment are the major driving factor for macrophage polarization. However, studies have demonstrated that nanoparticles can modulate macrophage polarization into different phenotypes. It was previously demonstrated that CoCrMo nanoparticle-induced macrophages (MCA) exhibit a difference in cytokine and chemokine expression than M1 and M2 macrophages, suggesting the possibility of a novel macrophage population. In this study, we performed mass spectroscopy to investigate the variation in macrophage phenotype induced by CoCrMo nano and microparticles and compare it with M1 and M2 macrophages. We hypothesize that, MCA macrophages polarized by CoCrMo particles will induce a distinct protein expression profile based on the particle size in comparison to M1 and M2 macrophages. The mass spectroscopy results support our hypothesis that MCA macrophages exhibit differential phenotypic characteristics based on the size of the particles. MCA-1 holds a unique position compared to other groups suggesting a significant variation in protein expression. |