| About this Abstract |
| Meeting |
2020 TMS Annual Meeting & Exhibition
|
| Symposium
|
Bio-Nano Interfaces and Engineering Applications
|
| Presentation Title |
Monitoring Interaction of Nanomaterials with Biomolecules and Their Dosimetry at Single Cell Level |
| Author(s) |
T. Venus, T. Meyer, J. Böttner, C. Merker, V, Calcagno, J. Keller, R. Landsiedel, D. Schwotzer, O. Creutzenberg, M. Moro, S. Moya, J. P. Longo, L. Muehlmann, Irina Estrela-Lopis |
| On-Site Speaker (Planned) |
Irina Estrela-Lopis |
| Abstract Scope |
The degree and the mechanism of uptake, localization and distribution of nanomaterials (NMs) in cells and organs are major issues concerning toxicity and risk assessment of the novel products. Furthermore, the application of NMs as devices for diagnostic and therapeutic purposes requires monitoring of their interaction with biomolecules.
The translocation of NMs across plasma membranes, their quantification and interaction with metabolic relevant cellular elements and biomolecules were studied in in vitro as well in vivo by means of label-free imaging methods based on element and molecule analysis. A quantitative analysis of minor and trace elements on the surface of NMs within single cells were performed and correlated with the cellular adverse response. Furthermore monitoring NM uptake, their bioprocessing, interaction with biomolecules and induced biochemical changes as well diagnostic and prediction of adverse effects were studied in living cells by means of confocal Raman microspectroscopy, cluster analysis or principle component analysis. |
| Proceedings Inclusion? |
Planned: Publication Outside of TMS |