| Abstract Scope |
Photocrosslinkable polymers such as gelatin methacrylate (GelMA) have been widely utilized in various 3D bioprinting application. These polymers crosslink when exposed to UV radiation in the presence of a photoinitiator. However, the photoinitiator may have negative effects on cell viability when the cell-laden bioink is used for printing. This study investigates the effects of photoinitiators on the cell viability in 3D bioprinting. The bioink contains 5% (w/v) gelatin methacrylate and 0.3-0.9% (w/v) photoinitiators. Two different photoinitiators, Irgacure 2959 and lithium phenyl-,2,4,6-trimethylbenzoylphosphinate (LAP), are used to compare different effects on cell viability during and after 3D bioprinting. During 3D bioprinting, cell viability is measured every 15 minutes within one hour. After crosslinking, the cell viability is measured after 0, 6, 12, and 24-hour incubation. The results show the significantly different effects of these two photoinitiators on cell viability in 3D bioprinting. |