| Abstract Scope |
The purpose of this study was to construct a composite cell-laden dual-layer hydrogel conduit with different functional layers as a three-dimensional scaffold. Here, we developed a modified solvent casting-based method to prepare gelatin methacrylamine/polyethylene glycol diacrylate (GelMA-PEGDA)-based dual-layer conduit seeded with mesenchymal stem cells. Through the pre-designed self-made mold, the inner and outer layer materials were injected into the predetermined space step by step, and supplemented with the steps of cooling and UV exposure process to complete the solidification of the material. Characterization results of morphology, and mechanical properties, and cell behavior test indicated that the two layers of the conduit are closely adhered and combined, and the conduit have good mechanical properties, suitable elasticity, and good biological properties. The cell-laden dual-layer hydrogel conduit and the manufacturing method are expected to be widely used in the field of tubular tissue repair and regeneration. |