About this Abstract |
| Meeting |
MS&T24: Materials Science & Technology
|
| Symposium
|
Society for Biomaterials: Biological Response to Materials and Material’s Response to Biological Environments
|
| Presentation Title |
Label-Free Measurement of Cell Viability in Hydrogel Scaffolds Using Optical Coherence Tomography |
| Author(s) |
Carl Simon |
| On-Site Speaker (Planned) |
Carl Simon |
| Abstract Scope |
A label-free method to measure cell viability in scaffolds was developed using optical coherence tomography (OCT). OCT is a reflective modality that uses infrared light to generate image contrast from changes in refractive index (RI). OCT can measure cell viability in 3D by detecting intracellular motions of organelles in live cells. When imaged by time series OCT, live cells demonstrate dynamic speckling, while dead cells do not, due to a lack of intracellular motions. Hydrogel samples containing either live or dead cells were imaged by time series OCT and intracellular changes in refractive index were used to identify live cells. Live cell counts from “live” samples showed a 13% difference from the expected number of live cells as determined from the known seeding density. “Dead” cell samples had only background levels of live cells. Further, the live cell positions were used to map the 3D cell distribution in the hydrogels. |