About this Abstract |
| Meeting |
2026 TMS Annual Meeting & Exhibition
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| Symposium
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Biomaterials for 3D Printing of Scaffolds & Porous Materials
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| Presentation Title |
Investigation of Chemo-naive and Chemo-resistant Ovarian Cancer Progression Behavior using a 3D Bioprinted Tumor Microenvironment Model with Perfusion |
| Author(s) |
Md Shahriar, Marielena Molinares Ponce, Ganesh Acharya, Komaraiah Palle, Changxue Xu |
| On-Site Speaker (Planned) |
Md Shahriar |
| Abstract Scope |
Cancer metastasis involves tumor cell detachment, migration through vessels, and distant colonization, driven by 3D interactions with the extracellular matrix (ECM) and complex microenvironments. Traditional 2D cultures and 3D spheroid models lack vascular flow, limiting their ability to mimic these conditions. We developed a 3D tumor microenvironment model by encapsulating SKOV3 ovarian cancer cells in gelatin methacrylate (GelMA) hydrogels with a perfusable microchannel created using sacrificial Pluronic F-127. Cells were cultured under static and perfused conditions for seven days, analyzed across three radial zones (0–300 µm, 300–600 µm, 600–900 µm) from the channel. Perfusion enhanced proliferation, migration, and viability compared to static conditions, with values decreasing radially. Carboplatin perfusion revealed chemo-naïve cells had reduced proliferation and viability at higher doses, while chemo-resistant cells showed greater drug tolerance. This model recapitulates key metastatic features, providing a platform to study cancer progression and drug responses. |
| Proceedings Inclusion? |
Planned: |
| Keywords |
Additive Manufacturing, Biomaterials, |