About this Abstract |
| Meeting |
MS&T26: Materials Science & Technology
|
| Symposium
|
3D Printing of Biomaterials and Devices
|
| Presentation Title |
Biofabricated Secretome-Enabled Scaffolds and Vessel-on-a-Chip Platforms for Skeletal Tissue Engineering in Diabetes |
| Author(s) |
Indranath Mitra, Josh Levin, Namitchandra Nallapaneni |
| On-Site Speaker (Planned) |
Indranath Mitra |
| Abstract Scope |
Diabetes mellitus (DM) impairs skeletal tissue regeneration by disrupting stem cell function and microenvironmental signaling. This work presents an integrated biofabrication strategy combining bioactive 3D-printed scaffolds with a biomimetic vessel-on-a-chip platform to address these challenges. Stem cell–laden GelMA/PEGDA bioinks were functionalized with endothelial and macrophage-derived secretomes to prime the cellular niche and guide stem cell fate under diabetic conditions. Concurrently, a microfluidic vessel-on-a-chip system incorporating a semi-permeable hydrogel barrier was developed to recapitulate sinusoidal capillary transport characteristic of bone marrow, enabling physiologically relevant glucose diffusion. This platform provides a dynamic testing environment to evaluate scaffold performance under transport-controlled diabetic conditions. By coupling secretome-mediated niche engineering with biomimetic nutrient transport, this approach aims to enhance stem cell localization, survival, and regenerative potential. Together, these biofabricated systems establish a foundation for advanced in vitro modeling and precision biomaterial design for diabetes-associated skeletal tissue repair. |