About this Abstract |
| Meeting |
2027 TMS Annual Meeting & Exhibition
|
| Symposium
|
Advanced Biomaterials and Implants
|
| Presentation Title |
Epigenetic Modulation and Gene Expression in Pre-Osteoblasts Induced by Micro-Arc Oxidation-Derived Coatings |
| Author(s) |
Mariana Rocha Corręa, Célio Junior da Costa Fernanndes, Kátia Barbaro, Karine Stefany Coan, Julietta Rau, Diego Rafael Nespeque Correa, Carlos Roberto Grandini |
| On-Site Speaker (Planned) |
Mariana Rocha Corręa |
| Abstract Scope |
Titanium alloys are widely used in load-bearing orthopedic implants; however, the release of metallic ions has motivated the development of surface modification strategies. In this study, the alloy surface was functionalized by micro-arc oxidation (MAO) using electrolytes enriched with calcium, phosphorus, and transition metal oxides. The resulting coatings were evaluated for cytocompatibility, osteogenic potential, epigenetic modulation, and antimicrobial activity. None of the surfaces exhibited cytotoxic effects. In MC3T3-E1 cells, the coatings upregulated the expression of adhesion- and proliferation-related genes (CDK2 and CDK4) and modulated the 5hmC/5mC ratio in the Nfkb promoter, indicating epigenetic reorganization. In adipose-derived mesenchymal stem cells (aMSCs), calcium- and phosphorus-rich surfaces enhanced mineralization, while the coating containing transition metal oxides (3 g/L) inhibited the growth of Staphylococcus aureus by more than 44%. These findings demonstrate that MAO functionalization generates multifunctional surfaces with promising potential for load-bearing orthopedic implant applications. Financial Support: CNPq and FAPESP. |
| Proceedings Inclusion? |
Planned: |