About this Abstract |
| Meeting |
MS&T25: Materials Science & Technology
|
| Symposium
|
Next Generation Biomaterials
|
| Presentation Title |
Super-Resolution Live Imaging of Cells in Confined Microspaces Using 3D CYTOP Microfluidic Chips Fabricated by Femtosecond Laser |
| Author(s) |
Koji Sugioka, Mirai Hanzawa, Kotaro Obata, Masatoshi Takagi, Asako Sakaue-Sawano, Asako Tosaki, Satoshi Shimozono, Felix Sima, Hiroyuki Kawano, Takuro Tojima, Daisuke Miyashiro, Akihiko Nakano, Atsushi Miyawaki |
| On-Site Speaker (Planned) |
Koji Sugioka |
| Abstract Scope |
Live imaging of cellular dynamics in confined areas is crucial for understanding biological processes including cancer cell invasion, immune response, and neuronal development. However, conventional glass- or PDMS-based microfluidic chips that provide such micro/nano-environments suffer from image distortion due to refractive index mismatch with water-based culture media, making it difficult to capture high-resolution images. To address this problem, we have developed a technique fabricating three-dimensional microfluidic chips using fluoropolymer CYTOP, because its refractive index (~1.34) closely matches that of water (~1.33). Our fabrication approach combining two-photon polymerization of SU-8 with mold processing enabled creating defect-free, flexible 3D structures with sub-micrometer precision. The fabricated CYTOP microfluidic chips realized super-resolution imaging of cancer cells in the microchannels and clearly visualized the rupture and repair of nuclear envelope during the migration. The ability of super-resolution imaging in microenvironments expands opportunities for investigating many biological studies other than cancer cell migration study. |