About this Abstract |
| Meeting |
2026 TMS Annual Meeting & Exhibition
|
| Symposium
|
Advanced Biomaterials and Implants
|
| Presentation Title |
A Bioengineered Tubular Scaffold Platform for in Vitro Modeling of Renal Proximal Tubule Structure and Function |
| Author(s) |
Anjali Sudha, Amrita Natrajan, Kyra Ramirez, Vineeth Viijayan, Derrick Dean |
| On-Site Speaker (Planned) |
Derrick Dean |
| Abstract Scope |
This study investigates the development and characterization of polyurethane (PU)-based electrospun tubules as an in vitro model for renal proximal tubules, with surface modifications using tetraethoxy silane (TEOS) based plasma vapor deposition and type 1 collagen coatings. Attachment and spreading of renal proximal tubule epithelial cells (RPTECs) on the coated tubules indicated strong cell-substrate interactions with stable adhesion. Immunostaining confirmed the expression of renal-specific markers, including Aquaporin 1 (AQP1) and Collagen IV, verifying the maintenance of a proximal tubule epithelial phenotype. The brush border enzyme-alkaline phosphatase activity (ALP) was significantly higher (p ≤ 0.05) in plasma and collagen-coated tubules over a 14-day period (3.4 fold) indicating enhanced renal tubular functionality. These results demonstrate that both plasma and collagen coatings improved the performance of electrospun PU tubules as a functional model for renal epithelial cells, with collagen coatings providing the most favorable environment for cell adhesion, function and renal-specific marker expression. |
| Proceedings Inclusion? |
Planned: |
| Keywords |
Biomaterials, Polymers, Other |