About this Abstract |
| Meeting |
2026 TMS Annual Meeting & Exhibition
|
| Symposium
|
Bio-Nano Interfaces and Engineering Applications
|
| Presentation Title |
Reversible Esterfication of Horseradish Peroxidase with Poly(aminophenylboronic acid) Nanorods Coated on Flexible Polypropylene Membranes |
| Author(s) |
Kayla Lenz, Hussian Maanaki, Terry Xu, Jun Wang |
| On-Site Speaker (Planned) |
Kayla Lenz |
| Abstract Scope |
We present the development of poly(aminophenylboronic acid) nanorod (PABA-NR) coated polypropylene (PP) membranes for reversible capture of glycoproteins. Boronate affinity materials (BAMs) like PABA-NRs undergo pH-dependent reversible esterification with glycoproteins. These interactions are frequently analyzed in solution—a dynamic and idealized environment that fails to represent the immobilized conditions required for solid nano-bio interfaces. Reversible esterification with membrane-bound PABA-NRs provides a more realistic demonstration of BAM effectiveness. The membrane should achieve capture/release comparable to solution-based testing, while maintaining coating stability. Therefore, PABA-NR synthesis was optimized to obtain uniform nanorods capable of surrounding PP fibers for enhanced attachment. Furthermore, the coating process was refined through various PP pretreatments and film application techniques. Horseradish peroxidase served as the model glycoprotein, showing robust capture at physiological pH and successful release under mildly acidic conditions. This reversible glycoprotein capture from a flexible and low-cost PP membrane demonstrates applicability in biosensing, glycoprotein purification, and therapeutics. |
| Proceedings Inclusion? |
Planned: |
| Keywords |
Nanotechnology, Polymers, |