About this Abstract |
| Meeting |
MS&T25: Materials Science & Technology
|
| Symposium
|
Nanotechnology for Energy, Environment, Electronics, Healthcare and Industry
|
| Presentation Title |
Synthesis and Characterization of Flexible Poly(aminophenylboronic acid) Nanorods for Reversible, High Affinity Glycoprotein Capture at Physiological Conditions |
| Author(s) |
Hussian Maanaki, Kayla Lenz, Terry Xu, Jun Wang |
| On-Site Speaker (Planned) |
Hussian Maanaki |
| Abstract Scope |
We present poly(aminophenylboronic acid) nanorods (PABA-NRs) for capture/release of glycoproteins, including: horseradish peroxidase (HRP), SARS-CoV-2 spike protein (SP), acetylcholinesterase (AChE), and glycated hemoglobin (HbA1c). Boronate affinity materials like PABA function as synthetic analogues to biological receptors, enabling enrichment and reversible esterification with glycoproteins. Successful enrichment requires maximizing affinity while minimizing protein degradation—two often conflicting conditions, since boronic acids typically have a pKa above physiological pH. PABA-NRs address this via secondary amines that lower the pKa and multivalent binding sites. Synthesis conditions were optimized, achieving uniform nanorods (3-8 µm long, 50-80 nm diameter) and high yield. PABA-NRs successfully captured HRP at physiological pH, with a dissociation constant <1.77 pM. Reversibility was confirmed with minimal capture below the pKa of 5.3, and full capture at pH>6. Similar results were achieved for SP, AChE, and HbA1c, highlighting the potential of PABA-NRs for reversible, high-affinity capture of glycoproteins under physiological pH. |