About this Abstract |
| Meeting |
MS&T24: Materials Science & Technology
|
| Symposium
|
Society for Biomaterials: Biomaterial Applications
|
| Presentation Title |
Thermally Responsive Microswimmers Biomanufactured by Genetically Engineered Probiotics for Antibiofilm Therapies |
| Author(s) |
Prakriti Dhungana, Jonathan Caguiat, Byung-Wook Park |
| On-Site Speaker (Planned) |
Prakriti Dhungana |
| Abstract Scope |
Microbes, such as Staphylococcus aureus, form biofilms when they cause infections. These are aggregates of bacteria embedded in an extracellular polysaccharide matrix. Microswimmers are promising biohybrid material platforms for biomedical applications. Herein, we report a new biohybrid material platform and method to biomanufacture thermal responsive microswimmers composed of genetically engineered probiotic Escherichia coli Nissle 1917 (EcN) and nanoheating components. We discuss the design and optimization of the biotic and abiotic components of the microswimmers. The gene for polysaccharide depolymerase (dispersin B, dspB) was cloned under the control of a heat shock promoter and transformed into EcN. Upon thermal localized heating of the biohybrid material platform embedded with EcN carrying the dspB construct, EcN produce dspB. The enzyme activity and biofilm assay are conducted to validate the effectiveness of the expressed dspB. We anticipate the thermal responsive microswimmers to enhances the efficiency of antibiofilm treatment by the enzymatic degradation of polysaccharides. |