Bio-Nano Interfaces and Engineering Applications: Bio-Nano Interfaces III
Sponsored by: TMS Functional Materials Division, TMS Structural Materials Division, TMS: Biomaterials Committee
Program Organizers: Candan Tamerler, University of Kansas; Kalpana Katti, North Dakota State University; Hendrik Heinz, University of Colorado Boulder; Terry Lowe, Colorado School of Mines; Po-Yu Chen, National Tsing Hua University
Thursday 8:30 AM
February 27, 2020
Room: Vista
Location: Marriott Marquis Hotel
Session Chair: Kalpana Katti, North Dakota State University; Terry Lowe, Colorado School of Mines
8:30 AM Invited
Functional Nanomaterials and Their Applications in the Therapy of Cancer and Infectious Diseases: Hanene Belkahlac1; Rym Boudjemaab2; John Lomas3; Olivier Micheauc1; Claire Wilhelm4; Hyacinthe Randriamahazaka3; Miryana Hemadi3; 1Université de Bourgogne Franche-Comté; 2Abbelight; 3Université de Paris - ITODYS Laboratory; 4Université de Paris
Multifunctional nanomaterials are finding applications in nanomedicine. In particular, magnetic and Carbon nanomaterials are extensively used in theranostics especially for imaging and treatment by thermal therapy. Firstly, bioimaging and photothermia(PT) were performed on E. coli, a Gram(-) bacterium, incubated with Carbon Dots(CDs). Remarkably, by PT, CDs are able to eradicate bacteria in their exponential and stationary phases. Images obtained by 3D super-resolution fluorescence microscopy clearly show the CD distributions in surviving bacteria after mild photothermal treatment. Secondly, In order to optimize the therapeutic efficacy and to enhance the targeting abilities in a cell cancer line, iron oxide nanoclusters were functionalized by different proteins: TRAIL, Transferrin and HSA. The efficiency was compared with magnetic hyperthermia or PT. An original mechanism was established that implies hotspot generation around the nanoclusters and, therefore, at the cell surface in the vicinity of the targeted-receptors, leading to disruption of the membrane and subsequent cell death.
9:00 AM
AMP-loaded Mesoporous Strontium Silicates with Improved Bioactivity and Antibacterial Capability: Isha Mutreja1; Kami Hogan1; Dhiraj Kumar2; Conrado Aparicio1; 1Minnesota Dental Research Center for Biomaterials and Biomechanics; 2Division of Pediatric Dentistry, University of Minnesota
We synthesized multifunctional mesoporous silicate nanostructures (MSNs): Ca-MSN, Sr-doped CaMSNs (Sr-CaMSNs) and novel Sr-MSNs via sol-gel method for bone regeneration and as antimicrobial peptide (AMP) cargo delivery nano-system. MSNs were characterized for cytotoxicity against mesenchymal stem cells as well as chemically and structurally using scanning electron miscroscopy with energy dispersive X-ray spectroscopy and wide-angle x-ray diffractometry. Peptide loading and release from MSNs was assessed using the fluorescent-labelled GL13K AMP. Ca-MSNs and Sr-CaMSNs were 200±50nm in diameter, and Sr-MSNs were 400±50nm. All MSNs had the expected composition, were amorphous, and had similar peptide-loading efficiency (96 ± 1%) with slow release profile. Sr-MSNs and Ca-MSNs released 7% and 4% of loaded AMP in 72h, respectively. MSNs improved cell metabolic activity, which confirmed their good cytocompatibility. In conclusion, Sr-MSNs support cell growth over extended periods and prolonged AMP release showing potential application for bone regeneration with antimicrobial potency.
9:20 AM
Application of Titanium Dioxide Nanotubes and Lattice Light-sheet Microscopy in Establishing Early-stage Cellular Response Mechanisms: Jevin Meyerink1; Scott Wood1; Jeremy Mercuri2; Robert Anderson1; Brandon Scott1; Grant Crawford1; 1South Dakota School of Mines & Technology; 2Clemson University
There is a need for novel titanium surface treatments, like TiO2 nanotubes, to promote osteogenesis on orthopedic implants, yet, the exact mechanisms by which this nanostructure influences adhesion dynamics and how those dynamics subsequently mediate intracellular signaling pathways to dictate cell function, remains unclear. The purpose of this study is to use advanced live-cell imaging techniques to understand how nanostructured surfaces influence early-stage cell adhesion. We investigate the influence of TiO2 nanotube structure on initial cell-TiO2 nanotube interaction of various cell types (pre-osteoblasts, macrophages, mesenchymal stem cells) via lattice light sheet microscopy (LLSM). Taking advantage of recent advances in LLSM, we are capable of rapid volumetric image acquisition during early-stage adhesion and long imaging duration (hours to days). In addition, we evaluate the requirement for specific proteins in the nanotube diameter-modulation of osteogenic and phagocytic signaling dynamics, with the goal of correlating these important dynamic processes to TiO2 nanotube diameter.
9:40 AM Invited
Nano-engineered DNA based Hydrogel as a New Injectable Drug Delivery Platform: Arghya Paul1; Sayantani Basu2; Settimio Pacelli2; 1The University of Western Ontario; 2University of Kansas
Injectable hydrogels present several advantages over pre-fabricated scaffolds including ease of delivery, shear thinning property and broad applicability in the fields of drug delivery and tissue engineering. Here we report development of a novel injectable hydrogel, composed of DNA and silicate nanodisks, as a drug delivery platform for orthoregeneration applications. Precisely, the hydrogel was designed by combining two different type of networks: a first network made of interconnections between neighboring DNA strands and a second one consisting of electrostatic interactions between the nanodisks and the DNA backbone. The silicate nanodisks were introduced to increase the viscosity of the DNA physical hydrogel and improve their shear-thinning properties. Additionally, the hydrogel network properties can be tailored to modulate the drug release kinetics, as per need. In vivo experimental results confirm that the hydrogel can efficiently deliver osteoinductive drugs and promote bone repair in rat model with critical size cranial bone defect.
10:10 AM Break
10:25 AM
Attachment Surface Energy as an Indicator for Adhesion of Nitrifying Bacteria: Ting-Ting Chang1; Po-Yu Chen2; 1Industrial Technology Research Institute; 2National Tsing Hua University
In recent years, submerged biofilm systems are progressively used in wastewater treatment and the attachment capability of bacteria on biocarriers is one of the crucial design factors. In this work, we used atomic force microscope (AFM) to study bacteria attachment on different types of biocarriers and for verifying systematically physicochemical properties. Ammonia-oxidizing bacteria (Nitrosomonas europaea) was selected and fixed on the probe of AFM for the attachment force measurements with varying surface energies, interfacial energies, and functional properties. The results showed that biocarrier possessed adhesion forces of 1.95 mJ/m2 and flexural modulus of ~300 MPa. In addition, the experiment showed a good correlation between attachment forces and attachment surface energies. This work demonstrated that AFM can be successfully applied as a platform to fast screen and determine the materials selection of biocarrier based on corresponding bacteria and provided potential strategies for improving treatment performance through optimized biocarrier material designs.
10:45 AM Invited
Biological Fate of Engineered Nanomaterials: Tracing Aggregation/degradation and Nanomaterial Dose In-vitro and In-vivo: Sergio Moya1; 1CIC biomaGUNE
There is an urgent need for a deeper understanding of the impact of engineered nanomaterials (ENMs) on human health resulting from deliberate exposure to ENMs, such as in nanomedicine, or from accidental exposure. The physical characteristics of ENMs determine their interaction with biomolecules and the ENMs fate both intracellularly and at body level. Several aspects of ENMs fate will be discussed in relation with ENM quantification and stability. Protein corona formation and stability, and the aggregation behavior ENMs will be investigated by means of Fluorescence Correlation Spectroscopy (FCS) in live cells along with ENMs trafficking. The bio distribution, organ accumulation and fate of radiolabelled ENMs will be studied in animal models by means of Positron Emission Tomography (PET). A dual radiolabelling strategy of core and coating will be presented using gamma emitters. Energy-discriminant Single-Photon Emission Computerised Tomography (SPECT) traces independently both labells and is used to evaluate NP integrity.
11:15 AM Cancelled
Biomimetic Nanointerface Functionalized Microchip for Highly Selective and Efficient Enrichment of Circulating Tumor Cells: Chaoyong Yang1; Lingling Wu2; 1Xiamen University; 2Shanghai Jiao Tong University
The study on circulating tumor cells (CTCs) has great significance for cancer diagnosis, prognosis, and personalized therapy. Herein, a biomimetic membrane-nanointerface enabled multivalent microfluidic chip (BioMem-Chip) was designed to selectively enhance the interaction of CTCs and capture substrate for highly efficient and high purity capture of CTCs. The deterministic lateral displacement-patterned microfluidic chip afforded selective frequency-enhanced collision of CTCs to micropillars owing to the larger size of CTCs over blood cells. The microchip substrate controllably assembled with leukocyte membrane nanovesicles offered convenient and efficient modification of multi-valent aptamers. Moreover, the obtained biomimetic membrane nanointerface selectively enhanced binding affinity to CTCs, owing to the multivalent aptamer–antigen binding and its natural blood cell resistant capability. Therefore, compared with other microdevices, our BioMem-Chip overcomes the limitation of capture performance tradeoff between high efficiency and high purity, possessing great potential in CTC clinical application and in-depth analysis.
11:45 AM
Inkjet Printed Nano-patterned Aptamer-based Sensors for Improved Optical Detection of Foodborne Pathogens: Lixby Diaz-Amaya1; Min Zhao1; Li-Kai Lin1; Carlos Ostos2; Jan Allebach1; George Chiu1; Amanda Deering1; Lia Stanciu1; 1Purdue University; 2Universidad de Antioquia
The increasing incidence of infectious outbreaks imposes a global burden for food safety, creating a market demand for on-site, disposable, easy-to-use and cost-efficient devices. This work aims to provide a cost-efficient, reliable and repeatable approach, for the detection of foodborne pathogens in real samples. We propose for the first time an optimized ink-jet printing platform, using a controlled nano-patterning of novel carboxyl-functionalized aptameric ink on nitrocellulose substrate for the highly efficient detection of E. coli O157:H7 (25 CFU ml-1 in pure culture and 233 CFU ml-1 in ground beef). This technology demonstrates the ability to control the variation within +/-1SD for at least 75% for data collected even at very low concentrations. To the best of our knowledge, this work reports the lowest LOD of the state-of-the art for paper-based optical detection of E. coli O157:H7, with enough evidence (p > 0.05) to prove its high specificity.