Additive Manufacturing and Cellular/Lattice Structures: Designs, Realization and Applications: Poster Session
Sponsored by: TMS Additive Manufacturing Committee, TMS Materials Characterization Committee
Program Organizers: Li Yang, University of Louisville; Allison Beese, Pennsylvania State University; John Carpenter, Los Alamos National Laboratory; Carolyn Seepersad, Georgia Tech; Miguel Aguilo, Morphorm LLC
Monday 5:00 PM
October 10, 2022
Room: Ballroom BC
Location: David L. Lawrence Convention Center
A-1: Cytotoxicity of Strontium Calcium Polyphosphate on MC3T3-E1 Cells in 3D Printed Alginate/Collagen Scaffolds: Sally Lee1; Serin Ahn1; Chris Mathew1; Abdulhadi Badran1; Shebin Tharakan1; Shams Khondkar1; Michael Hadjiargyrou1; Azhar Ilyas1; 1New York Institute of Technology
Alginate and collagen are cell-compatible biomaterials that foster spontaneous proliferation. Prior studies have shown strontium calcium polyphosphate (SCPP) as a suitable crystalline compound to enhance bone formation in osteoblasts. Concentrations of 1% SCPP have shown the greatest bone growth compared to higher cytotoxic concentrations. Our study utilizes 1% SCPP in a dose-dependent manner to evaluate cytotoxicity on MC3T3-E1 cells in Alginate/Collagen composites in-vitro. MC3T3-E1 cells were seeded in at 1,000 cells/well with doses of 2 to 20 mg of only 1% SCPP. Cytotoxicity was observed at 20 mg, likely due to a low pH of the SCPP resulting in severe cytotoxicity. We hypothesize 3D bioprinted cell-laden scaffolds will demonstrate greater proliferation and less cytotoxicity due to alginate’s inherent rigid properties and collagen’s cell-binding sites. The results of cell-surface interactions of these scaffolds for cell growth, proliferation, and the mineralized tissue formation in-vitro will also be presented and discussed.