Advanced Real Time Imaging: Bio-Nano Interfaces and Engineering Applications (Joint Session)
Sponsored by: TMS Functional Materials Division, TMS: Advanced Characterization, Testing, and Simulation Committee, TMS: Alloy Phases Committee
Program Organizers: Jinichiro Nakano, MatterGreen; David Alman, National Energy Technology Laboratory; Il Sohn, Yonsei University; Hiroyuki Shibata, Tohoku University; Antoine Allanore, Massachusetts Institute of Technology; Candan Tamerler, University of Kansas; Noritaka Saito, Kyushu University; Neslihan Dogan, TU Delft; Zuotai Zhang, Southern University of Science and Technology; Bryan Webler, Carnegie Mellon University; Anna Nakano, US Department of Energy National Energy Technology Laboratory

Tuesday 2:00 PM
February 25, 2020
Room: Theater A-4
Location: San Diego Convention Ctr

Session Chair: Candan Tamerler, University of Kansas; Jinichiro Nakano, MatterGreen


2:00 PM  
Micro-scale Imaging of Cancerous Tissues using High Frequency Ultrasound: Leila Ladani1; Koushik Paul1; 1Arizona State University
    Imaging tissues and the ability to detect micro-scale cellular variations is the next step in detecting early stages of cancer. However, at the present, this capability is not available. High frequency ultra sound method was previously shown to have the ability to detect micro-scale inclusions in tissue phantoms. The analysis has shown increased sensitivity to phantom thickness and the inclusion size. This study is conducted on tissue phantoms with different scatter size to vary the scattering regime from Rayleigh to stochastic regime. It is found that volume ratio of the scatter also impacts the results. With the increase in size and quantity of the scatter, the number of peaks increases representing the abnormality level in the phantom micro structure. Computational modeling of high frequency ultrasound passing through micro-scale tissue phantoms containing scatter confirms the fundamental understanding of the scattering mechanism.

2:20 PM  
New Biomarkers of Prostate and Breast Cancer Metastasis to Bone: Kalpana Katti1; Sumanta Kar1; Haneesh Jasuja1; MD Shahjahan Molla1; Dinesh Katti1; 1North Dakota State University
    A novel cancer metastasis in vitro model was built using tissue-engineering approaches with nanoclay scaffolds that enable proliferation and differentiation of human mesenchymal cells to form hierarchical structures mimicking remodeling bone. Both prostate and breast cancer have the propensity to metastasize to bone thus causing fatalities. Cancer cell lines are seeded on the newly formed bone like structures. Gene and protein expression studies indicate that the tumors created in vitro are Mesenchymal to Epithelial transition(MET) of cancer metastasis. Nanoindentation is used obtain mechanical response of tumors. Softening of tumor cells at MET as well as softening at metastasis progression is reported. FTIR experiments indicate unique signatures of DNA and protein contents during cancer progression. Immunocytochemistry as well as gene expression studies indicate a vital role of cytoskeletal elements in the mechanics of cancer cells at tumor progression. The cancer testbed enables the discovery of novel markers for metastasis.

2:40 PM  
Particle Size and Zeta Potential of CBD/THC Nano-emulsions Present in Commercial Beverages: Vidumin Dahanayake1; Vinod Radhakrishnan1; James Soulek1; 1Anton Paar USA
    Cannabidiol (CBD) oil derived from either the hemp or marijuana plant, has exhibited remedial effects on anxiety, pain and insomnia among other ailments. CBD is commonly associated with the psychoactive constituent tetrahydrocannabinol (THC), which shares a similar molecular structure to CBD and is also present in the same plants. The bio-availability of these oils in their native form is limited due to their hydrophobic nature. Kinetic solubility and therefore bio-availability can be improved by incorporating these components in nano scale droplets as oil in water emulsions. There has been growing interest in utilizing these emulsions in commercially available CBD/THC beverages. Herein, we have investigated the particle size of several over the counter beverages to estimate their potential bio-availability. Additionally, the Zeta potential of these particles will also be determined to gain an insight into shelf life and long term stability in solution.

3:00 PM  
Inkjet Printed Electrochemical Aptasensor for Detection of Hg2+ in Organic Solvents: Lixby Diaz-Amaya1; Li-Kai Lin1; Renee DiNino1; Carlos Ostos2; Lia Stanciu1; 1Purdue University; 2Universidad de Antioquia
    Real-time detection of heavy metal traces from industrial waste is key to control any potential pollution by early intervention. Despite the promising performance of novel technologies, the chances to transfer the lab-scale platforms to successful commercialization stages are dramatically low. We propose for the first time the use of ink-jet printed gold electrodes as a reliable, stable and fully-scalable approach for aptamer-based detection of mercury (Hg2+) traces in water and organic solvents. The controlled fabrication process was demonstrated with an RCT variation within +/- 1 SD (RSD= 2.92%). The LOD reported is 0.01 ppm in water and enhanced sensitivity of 2-fold in organic solvents (LOD = 0.005 ppm), both in compliance with EPA regulation. To the best of our knowledge, this work reports for the first time a gold inkjet-printed impedimetric platform for aptamer-based detection of Hg2+ in organic solvents, showing promising viability for future prototyping and scaling-up.

3:20 PM  
Tunable Self Assembled Supramolecular Nanoprobes to Decipher Biomedical Events: Mathew Jaeschke1; Sarah VanOosten1; Philip Elrod1; Esra Yuca1; Candan Tamerler1; 1University of Kansas
    Controlled interactions at the biological-material interfaces have a vast amount of engineering and clinical applications that may lead to novel imaging, diagnostic, and therapeutic systems. Recently, we have explored combining multiple sensing and imaging modalities using metallic nanoparticles. Gold nanoparticles are among the common nanoparticles systems utilized in biomedical applications, ranging from targeted delivery of drugs to bioimaging and the monitoring of cells and tissues. By using gold-binding (AuBP) peptides conjugated to fluorescent proteins (GFP and DsRed) we can combine fluorescent imaging with metallic nanoparticles plasmonic responses. We investigated the wide range of applicability of the supramolecular nanoprobes under different protein and nanoparticles concentrations using localized surface plasmon resonance spectroscopy. The optimized bioactive nanoprobes were further investigated for their stability under physiological environments. We finally demonstrate their use as sensing modalities for monitoring inorganic ions for monitoring biomedical events.

3:40 PM Break

4:00 PM  
Removal of Chromium (VI) from Water onto Activated Carbon by Adsorption in Dynamic Mode: Naouel Hezil1; Mamoun Fellah1; Alex Montagne2; Alain Iost3; Aleksei Obrosov4; Sabine Wiess4; 1Abbes Laghrour Khenchela University, Algeria; 2Ecole d'arts et metiers ; 3Ecole d'arts et metiers; 4Department of Physical Metallurgy and Materials Technology, Brandenburg Technical University
    Hexavalent chromium pollution from industrial waste water is a serious problem as it can cause adverse effects on the environment. Several methods are used to reduce the harmful effects of this pollutant, especially physico-chemical methods, such as adsorption technology. The present study aims to remove Cr(VI) from industrial sources in a fixed-bed column of activated carbon. The experiments were carried out at natural pH and temperature with a flow rate (5,10 and 20 mL/min) and bed height (3.5 Cm). Breakthrough curves for feed concentrations (0.01, 0.03 and 0.05 mol/L) were investigated. The results indicated a marked decrease up to 99%. The value of the flow constant for the Thomas model decreased with the increase in the concentration of the incoming substance, but increased with the increase in the flow rate.

4:20 PM  
Sc3N@C80 and La@C82 doped Graphene Photodetectors: Kishan Jayanand1; Ravindra Mehta1; Srishti Chugh1; Anupama Kaul1; 1University of North Texas
    High-performance hybrid graphene photodetectors were prepared with Sc3N@C80 and La@C82 deposited on graphene using electrophoretic deposition technique. The zero-dimensional (0D) metallofullerenes with two-dimensional (2D) graphene resulted in charge transfer processes to enhance the optoelectronic properties. The blue-shift (Δω(+)) in the Raman peaks of the G- and 2D-modes of the hybrids relative to bare graphene confirms that hole-doping is occurring in graphene with Sc3N@C80. In contrast, a red-shift (Δω(-)) in the Raman peaks confirms electron-doping occurs with La@C82. The charge transfer between graphene and metallofullerenes and consequent photocurrent generation over wavelength range of 400 to 1100nm contribute to a responsivity of ~109 AW-1 and ~108 AW-1 for the Sc3N@C80–Graphene hybrid and La@C82–Graphene hybrid, respectively. The hybrid systems also show a high Detectivity and External Quantum Efficiency. To the best of our knowledge, our results represent the first approach towards the fabrication of metallofullerene-graphene based photodetectors which does not require a complicated fabrication process and yields the highest reported values for photodetector device figures of merit. The exceptional performance gains achieved by the hybrid systems confirms the potential of metallofullerene doped graphene hybrid systems for a new class of 0D-2D photonic devices in the future for imaging, surveillance and defense-related application.