Bio-Nano Interfaces and Engineering Applications: Bio-Nano I
Sponsored by: TMS Functional Materials Division, TMS Structural Materials Division, TMS: Biomaterials Committee
Program Organizers: Candan Tamerler, University of Kansas; Kalpana Katti, North Dakota State University; Hendrik Heinz, University of Colorado Boulder; Terry Lowe, Colorado School of Mines; Po-Yu Chen, National Tsing Hua University
Monday 8:30 AM
March 15, 2021
Room: RM 12
Location: TMS2021 Virtual
Session Chair: Candan Tamerler, University of Kansas; KAlpana Katti, North Dakota State University
8:30 AM Invited
Bio-imaging with Photoluminescence of Single-layer MoS2: Yuhei Hayamizu1; 1Tokyo Institute of Technology
Visualizing biological activity in real time poses a novel way for biosensing. Single-layer Molybdenum disulfide (MoS2) shows strong photoluminescence (PL) under photo-excitation due to its semiconducting nature with direct bandgap. In this work, we demonstrate pH-sensitive photoluminescence of single-layer MoS2 grown by chemical vapor deposition (CVD). While CVD MoS2 grown from MoS2 powder did not have a strong pH sensitivity, MoS2 grown from MoO2 powder exhibited a sensitivity to the solution pH in its PL intensity. Next, we utilized the single-layer MoS2 as the active layer of an imaging sensor to monitor activity of individual bacteria by the MoS2 PL. For the demonstration, we employed Lactobacillus. It is one of the most studied probiotic bacteria which consumes glucose and produces lactic acid by fermentation. The bacteria were placed on a single-layer MoS2 and its PL intensity was monitored in real time.
9:00 AM Invited
Stickiness at Bio-nano-interfaces: From Nanoscale Characterization to Macroscale Properties: Hannes Schniepp1; 1College of William & Mary
In many cases, interactions at bio-nano-interfaces are weak van der Waals forces or other forms of “secondary” bonding. Nevertheless, biological materials often feature excellent structural properties, which is surprising. From the point of view of synthesizing bioinspired structural materials, this is very interesting, since this also means good materials can be made with a small energy footprint. Characterization of these weak interactions at the bio-nano-interface is thus important, but experimentally challenging. Furthermore, it is equally important and difficult to understand how such weak interactions translate into strong materials. We developed several experimental techniques using scanning probe techniques to quantitatively probe these interactions and study their impact on macroscopic material properties. These techniques are important to further our understanding of hierarchical biomaterials, as well as to develop synthetic, sustainable composites with outstanding properties.
9:30 AM Invited
Insight into the Mechanobiological Progression of Cancer Metastasis to Bone: Dinesh Katti1; Sharad Jaswandkar1; Kalpana Katti1; 1North Dakota State University
Cancer metastasis to bone is a complex biological phenomena that results in very poor prognosis for patients and occurs for both breast and prostate cancer. The bone metastasis phenomena and tumor formation is influenced by F-actins, the important constituents of the cytoskeleton of the cancer cell. We describe the use of a cancer testbed with tissue engineered bone scaffolds to experimentally evaluate the changes to the actin filaments during progression of cancer metastasis. We have developed multiscale computational models that describe the deformation mechanisms, mechanical properties, concentrations and localization of F-actin. Detailed molecular modeling of the deformation behavior of F-actin for a variety of loading paths show the important structural mechanisms that influence actin dynamics and hence cancer progression. The steered-molecular-dynamics simulations are guided by confocal imaging as well as cellular adhesion experiments on the metastasis testbed. The study shows mechanisms of F-actin dynamics during cancer progression at bone metastasis.
10:00 AM
Control of Scaffold Shear Forces Through a Perfusion Bioreactor for Design of Prostate Cancer Bone Metastasis Testbed: Haneesh Jasuja1; Akerkouch Lahcen1; Trung Le1; Dinesh Katti1; Kalpana Katti1; 1North Dakota State University
Prostate cancer (PCa) has a propensity to metastasize to bone, resulting in skeletal impairment and high mortality. Interstitial fluid flow induced mechanical cues are well known for their bone remodeling and cancer promigratory roles. However, the influence of mechanical signals PCa progression at bone metastasis is poorly explored. We developed a 3D in-vitro dynamic model to examine role of fluid derived shear stress on PCa progression to bone. We created a bone niche employing nanoclay based scaffolds and seeded human PCa cells on the tissue-engineered bone in a specially designed bioreactor to recapitulate PCa bone metastasis. Computational fluid dynamics studies suggest a high correlation between fluid velocity magnitude and scaffold pore size. The experimental results indicate uniform and directional distribution of hMSCs and varying PCa tumor morphology on dynamically cultured scaffolds. Thus, shear forces exerted by fluid flow can be used to influence metastasis characteristics of PCa.