BioNano Interfaces and Engineering Applications: Bionano Interfaces & Engineering Applications I
Sponsored by: TMS Functional Materials Division, TMS Structural Materials Division, TMS: Biomaterials Committee
Program Organizers: Candan Tamerler, University of Kansas; Hendrik Heinz, University of Colorado Boulder; Kalpana Katti, North Dakota State University; Terry Lowe, Colorado School of Mines; Po-Yu Chen, National Tsing Hua University

Tuesday 8:00 AM
March 1, 2022
Room: 201A
Location: Anaheim Convention Center

8:00 AM  Keynote
3D Printing for Bone Regeneration: Convergence of Knowledge: Susmita Bose1; 1Washington State University
    3D printing (3DP) or additive manufacturing (AM) is important in clinical needs for on-demand patient-matched implants due to better functionalities. Establishing process property relationships for different AM techniques is vital to their successful implementation in biomedical devices. AM of multiple materials in a single operation is also an exciting innovation. Hard biomaterials, e.g., calcium phosphate (CaP) ceramics being compositionally similar to the inorganic part of the bone, are often used in bone implants, as both 3DP tissue engineering scaffolds and surface modified hip and knee implant devices. We have used 3DP CaP scaffolds, for bone tissue engineering, where 3D interconnected channels in CaP scaffolds provide pathways for micronutrients transport and improved mechanical interlocking between scaffolds and surrounding bone. In vivo studies show improved osteogenesis, angiogenesis, and controlled drug delivery using natural medicinal compounds (NMCs) in these 3DP scaffolds and coatings demonstrating promise for their use in orthopedic and dental devices.

8:40 AM  Invited
NOW ON-DEMAND ONLY - Self-assembling Nanoclay Gels for Tissue Regeneration: Jon Dawson1; 1University of Southampton
    Clay nanoparticles offer a surprisingly rich array of opportunities for biomaterial design and regenerative medicine that are only beginning to be explored. In aqueous solutions nanoclay particles self-assemble into stiff gels upon contact with physiological ionic solutions. Upon implantation in the body, nanoclay gels provide bioactive environments, through the adsorption of proteins and ions that promote the invasion of cells. Due to their affinity for proteins such as growth factors, nanoclay gels can be applied to deliver, localise, enhance and even pattern activity of growth factors to initiate tissue regeneration. This talk will describe the development and application of nanoclays for growth factor delivery and regenerative medicine including recent work to translate this technology towards a therapy for bone fusion.

9:10 AM  
In vitro Pre-osteoblast Cell Differentiation by Luminescent Hydroxyapatite: Fabian Martinez1; Karla Juárez-Moreno2; Manuel Herrera2; Olivia Graeve1; 1University of California San Diego; 2Universidad Nacional Autónoma de México
    We present a differentiation study of murine pre-osteoblast cells in the presence of hydroxyapatite undoped and doped with rare-earth elements, including Eu2+, Eu3+, Ce3+, Tb3+, and Yb2+. Before assay studies, the hydroxyapatite powders were analyzed by X-ray diffraction and spectral cathodoluminescence, confirming that the dopants are present in the powders in amounts of a few atomic percent and they do not modify the equilibrium phase of hydroxyapatite. Alkaline phosphatase activity assay on the surface of treated cells, demonstrates that the incorporation of rare earth elements in the structure of hydroxyapatite modifies its effect upon cell differentiation. Moreover, to unravel whether the rare-earth elements drive an osteoconductive response, ongoing experiments are being completed to quantify the amount of differentiation gene biomarkers. We found that the incorporation of the species Eu2+, Eu3+, and Yb2+, into the crystal structure of the hydroxyapatite enhances significantly the differentiation of the pre-osteoblast cells.

9:30 AM Break

9:50 AM  
Sensing of COVID-19 Antibodies and Antigens in Seconds via Aerosol Jet Nanoprinted Reduced Graphene Oxide Coated Three Dimensional Electrodes: Md. Azahar Ali1; Chunshan Hu1; Sanjida Jahan1; Bin Yuan1; Mohammad Sadeq Saleh1; Fei Zhang2; Shou-Jiang Gao2; Rahul Panat1; 1Carnegie Mellon University; 2University of Pittsburgh Medical Center
    Rapid diagnosis is critical for the treatment and prevention of diseases. We report an advanced nanomaterial-based biosensing platform that detects antibodies and antigens specific to SARS-CoV-2 within seconds. The biosensing platform is created by 3D nanoprinting of a three-dimensional electrode, coating the electrode by nanoflakes of reduced graphene oxide (rGO), and immobilizing specific viral proteins on the rGO nanoflakes. The electrode is then integrated with a microfluidic device and used in a standard electrochemical cell. Limit-of-detection of 2.8 fM was achieved via this method and read by a smartphone-based user interface. Human tests are also carried out that validate this technique. The detection time of 11.5 seconds is achieved. The sensing is highly selective, and the proposed sensing platform is generic and can be used for the rapid detection of biomarkers for other infectious agents such as Ebola, HIV, and Zika, which will benefit the public health.