BioNano Interfaces and Engineering Applications: Bionano Interfaces & Engineering Applications II
Sponsored by: TMS Functional Materials Division, TMS Structural Materials Division, TMS: Biomaterials Committee
Program Organizers: Candan Tamerler, University of Kansas; Hendrik Heinz, University of Colorado Boulder; Kalpana Katti, North Dakota State University; Terry Lowe, Colorado School of Mines; Po-Yu Chen, National Tsing Hua University
Tuesday 2:30 PM
March 1, 2022
Room: 201A
Location: Anaheim Convention Center
2:30 PM
Calcium Phosphate-based Bone Grafts with Curcumin and Vitamin C Shows Osteogenic, Chemo-preventive and Anti-bacterial Potential for Orthopaedic and Dental Applications: Ujjayan Majumdar1; Susmita Bose1; 1Washington State University
Repair of critical-sized tumor-resection defects caused by severe infection, ageing and tumor ablation still remains a significant challenge in clinical orthopedics. Calcium phosphate based materials are of cardinal importance in bone tissue engineering applications due to their resemblance with human hard tissues. The objective of the study is to understand the effects of controlled release of curcumin and Vitamin C co-delivery system on the osteogenic, chemo-preventive and antibacterial properties of Calcium phosphate scaffolds. The antibacterial potential of this co-delivery system was assessed against S. aureus and the obtained results indicate that fabricated compositions are ~ 80 % antibacterial compared to control sample. Initial osteogenic property characterization indicates higher cell viability on the curcumin – vitamin C loaded scaffolds. This presentation will discuss the controlled release kinetics of Curcumin and vitamin C co-delivery system and their osteogenic, chemo-preventive and anti-bacterial potential, suitable for tissue engineering applications.
2:50 PM
Structural Development and Phase Transformation of Mesocrystalline Iron Oxides in the Ultrahard Teeth of Cryptochiton Stelleri: Taifeng Wang1; Wei Huang1; Wen Yang1; Satoshi Murata2; Atsushi Arakaki2; David Kisailus1; 1University of California, Irvine; 2Tokyo University of Agriculture and Technology
Many organisms synthesize complex, hierarchical, materials through mineralization processes under environmentally benign conditions. One such example are the ultrahard and wear-resistant radular teeth found in mollusks, which are used to scape against rock for food. Here, we reveal the biomineralization-controlled structural development of the hard, outer magnetite-containing shell of the teeth. We identify the formation of mesocrystalline iron oxides, initially consisting of ferrihydrite mesocrystals displaying an underlying spherulite-like morphology. In addition, we identified an interpenetrating organic matrix that likely consists of chitin-binding proteins with an affinity for iron sequestration. It is these organics that guide the nucleation and growth of the mesocrystalline oxide phase. These hierarchically arranged particles subsequently undergo a solid-state phase topotactic transformation to form mesocrystalline magnetite. Additional growth via Ostwald ripening leads to single crystalline octahedral magnetite particles around the mesocrystalline spherulite, which eventually yield continuous nanorods, which provide both strength and toughness to the mature teeth.
3:10 PM
Gingerol and EGCG Enhance Osteogenic and Antibacterial Properties of Ag-doped CaP Bone Grafts: Yongdeok Jo1; Susmita Bose1; 1Washington State University
As the world’s elderly population continues to grow rapidly, the number of orthopedic implant surgeries is increasing. During surgical intervention, the invasion of infectious agents frequently causes implant-related osteomyelitis. Antibiotics have been used to combat osteomyelitis in orthopedic applications. However, the high dose requirement, toxicity, and risk of treatment resistance have prompted interest in alternative multi-functionalized delivery systems with antibacterial and osteogenic properties. Silver (Ag) has provided antimicrobial properties to various microorganisms. E Epigallocatechin gallate (EGCG), the active compound in green tea leaves, and gingerol, a therapeutic compound extracted from ginger root, have shown the ability to enhance osteogenic properties and antibacterial properties. Our goal is to fabricate Ag-doped calcium phosphate (CaP) bone grafts with EGCG and gingerol to improve bone healing while providing infection prevention. Multifunctional Ag-doped CaP grafts with EGCG and gingerol have shown in vitro antibacterial properties to Staphylococcus aureus while enhancing in vitro osteoblast cellular activity.
3:30 PM
Zinc Functionalized Polydopamine-curcumin Coated 3D Printed Calcium Phosphate Scaffolds for Enhanced Osteogenesis, and in Vitro Chemoprevention: Arjak Bhattacharjee1; Susmita Bose1; 1W. M. Keck Biomedical Materials Research Lab,Washington State University
Osteosarcoma is the predominating bone malignancy which is the third most common cancer among pediatric patients and young adults. Since antiquity, curcumin, from turmeric are utilized in traditional Indian medicine (Ayurveda) to treat bone fractures and related disorders. The current work aims to utilize Zn functionalized polydopamine coating on 3D printed porous tricalcium phosphate (TCP) scaffolds for osteosarcoma inhibition. The binder jetting method was used to fabricate TCP scaffolds with interconnected porosity. Zn functionalized polydopamine coating over the scaffold surface was developed by controlled polymerization of dopamine in presence of zinc chloride. Zn and curcumin together help in osteoblast growth, proliferation, and increases the cell viability to ~ 1.5 times as compared to control. In addition, the presence of curcumin shows ~ 2 folds decrease in osteosarcoma cell viability after day 11. This presentation will discuss the fabrication of Zn functionalized polydopamine-curcumin-coated scaffolds and their osteogenic, chemo-preventive potential.
3:50 PM Break
4:10 PM Invited
Grain Size Effects and Mechanisms for Increased Antimicrobial Efficiency in Ultrafine-grained Bulk Copper: Evander Ramos1; Isabella Bagdasarian1; Yaqiong Li1; Masuda Takahiro2; Yoichi Takizawa3; Justin Chartron1; Alex Greaney1; Zenji Horita2; Joshua Morgan1; Suveen Mathaudhu1; 1University of California-Riverside; 2Kyushu University; 3Nagano Forging Co., Ltd
Copper and it’s alloys have long been known for their antiviral and antibacterial properties, and in the course of the global pandemic, research on ways to accelerate their effectiveness have come to the forefront. Recent studies have reported that when the crystal size of bulk copper reduces towards the nanoscale, the anti-viral and anti-microbial efficiency increases non-linearly. The mechanisms for this are diverse, and include the increase in volume fraction of ionic pathways to the surface, oxidation-reduction, topological features and others. In this study, we investigate the antibacterial characteristics of nanocrystalline pure copper produced by severe plastic deformation (SPD), and based on a literature review and our own emerging data, attempt to unravel the mechanisms that promote bacteria or cell destruction as a function of grain size. The versatility of SPD methods allows for the fabrication of bulk components that, unlike thin-film coating approaches, may enable scalability of medical applications.