BioNano Interfaces and Engineering Applications: Poster Session
Sponsored by: TMS Functional Materials Division, TMS Structural Materials Division, TMS: Biomaterials Committee
Program Organizers: Candan Tamerler, University of Kansas; Hendrik Heinz, University of Colorado Boulder; Kalpana Katti, North Dakota State University; Terry Lowe, Colorado School of Mines; Po-Yu Chen, National Tsing Hua University
Tuesday 5:30 PM
March 1, 2022
Room: Exhibit Hall C
Location: Anaheim Convention Center
K-14: AuNps Tailored Microneedle Based Point of Care Device Fabrication for Hormonal Sensing: Shubhangi Shukla1; 1NCSU
Leptin hormone levels are a vital biomarker for the glucose and fat metabolism in our body. The estimation of its concentration in blood is helpful for the prior diagnosis of chronic diseases. However, the process requires a selective and sensitive system for onboard detection in body fluids. Here, we intend to devise a noninvasive, eco-friendly, and imprint-free immunosensor based on ultrananocrystalline diamonds (UNCDs) microneedle array. The UNCDs surface is modified with an amphiphilic silane [(3-aminopropyltrimethoxysilane (3-APTMS)] functionalized gold nanoparticles (AuNPs) binded with monoclonal Anti-leptin receptor moieties for leptin transducers. The AuNPs were synthesized by chemical reduction method using 3-APTMS as a capping agent in the presence of formaldehyde as a reductant. The surface plasmons were coherently coupled with antibody molecules through glutaraldehyde and the silane effect on the electrode surface. Under NTP, the proposed immunosensor exhibited a wide linear range of 0.121–2500 pg/mL with a low detection limit of 0.025 pg/mL.
K-15: Potentiometric Immunosensor for the Selective Thyroxine Detection Using Cu-MOF@PANI Composite in Serum Samples: Mradula Mradula1; Rocky Raj1; Sunita Mishra1; 1CSIR-Central Scientific Instruments Organization
A label-free electrochemical immunosensor was fabricated for detection of thyroxine hormone by immobilizing anti-thyroxine monoclonal antibody (Ab) onto copper metal-oxide framework (Cu-MOF) and polyaniline (PANI) composite modified screen-printed carbon electrode (SPCE). Cu-MOF and their composite were characterized by field emission scanning electron microscopy (FESEM), Fourier transform infrared spectroscopy (FTIR), RAMAN spectroscopy, X-ray diffraction (XRD), and cyclic voltammetry (CV). The immunosensor possessed a dynamic linear range (10–105 pM) with a low limit of detection (LOD) of 0.33 pM (0.25 pg/ml) for CV. The evaluation study of immunosensor displayed a good analytical recovery of ≥ 90% when the different concentration of thyroxine was added in serum sample. In addition, to determine the reproducibility for working electrode between and within batch, the relative standard deviation calculated were 3.87% and 6.04%, respectively, represent it's good reproducibility. The immunosensor retained 90% of its initial activity after its regular use for 40 days, while being stored at 4 °C.