Bio-Nano Interfaces and Engineering Applications: Poster Session
Sponsored by: TMS Functional Materials Division, TMS Structural Materials Division, TMS: Biomaterials Committee
Program Organizers: Candan Tamerler, University of Kansas; John Nychka, University of Alberta; Kalpana Katti, North Dakota State University; Terry Lowe, Colorado School of Mines
Tuesday 6:00 PM
February 28, 2017
Room: Hall B1
Location: San Diego Convention Ctr
H-6: Development of Functional Peptides with β-sheet Structures for the Self-assembly on Two-dimensional Materials: Kohei Sakuma1; 1Tokyo Institute of Technology
Peptide self-assembly on two-dimensional (2D) materials has been studied, aiming to applications for biosensors. A problem in the usage of peptides is their instability under aqueous solutions. To improve it, amino acid sequences of previously reported peptides were relatively complex. Thus, it could be beneficial to design a peptide which form stable assembled structures with a simple sequence. In this work, we developed peptides inspired by Fibroin which is a protein makes strong β-sheet structures in a silk. The sequence for the self-assembly has only glycines and alanines. We have succeed to form self-assembled structures with linear ordered structures on 2D materials, such as Graphite, MoS2, and BN, and evaluated their stability in aqueous solutions.
H-7: Regeneration Sands Foundry for Deterioration Bacterial in Industrial Scale: Viviane Rodrigues1; Bruno Karolski1; Jorge Tenório1; 1University of São Paulo
The foundry sands has refractory characteristics for the preparation of molds and cores that form metal parts. And it is necessary to regularly dispose of a quantity of sand used equivalent to the amount of new sand purchased, and this contaminated sand is classified as "non - inert". The treatment method used in the research was the bacterial degradation, allowing the cost to be more economical, regardless of scale to be industrial. Also reducing the environmental impact, it is no longer necessary to reject the contaminated sand in a landfill. Bacteria are isolated in the laboratory and they use the contaminant as a carbon source, ie they digest the resin contaminates sand, degrading compound harmless to the environment since most of the synthetic resins is hostile to the ecosystem. The main results of the research are the absolute decontamination of sand casting, reducing process costs and degradation of synthetic resin.
H-8: Effect of Doped Magnesium in Titanium Nitride Coatings on Behavior of Mesenchymal Stem Cells: Sakip Onder1; Ayse Calikoglu-Koyuncu2; Kursat Kazmanli3; Mustafa Urgen3; Fatma Nese Kok3; Gamze Torun-Kose2; 1Isik University; 2Yeditepe University; 3Istanbul Technical University
Mg was doped in TiN thin film coatings by using arc-PVD technique to increase the biocompatibility and longevity of titanium implants and its effect on behavior of mesenchymal stem cells (MSCs) was studied. Increasing bias voltages (–600, –800 and –1000 V) were applied to heat the samples before coating and the samples were continuously rotated for homogeneous film formation. Secondly, MSCs isolated from the bone marrows of 6-8 weeks old Sprague-Dawley rats were seeded on the implants with a density of 2000 cells/sample. The media on the samples was replaced with osteogenic differentiation medium three days after cell seeding. Finally, characterization studies such as cell proliferation assay (MTS), collagen type I immunostaining and alkaline phosphatase (ALP) assay were conducted. Results showed that all surfaces provided good cell attachment and proliferation but collagen type I deposition and ALP activity was higher at Mg containing sample.
H-9: Determination of Cell Adhesion on Supported Lipid Bilayers by Quarz Crystal Microbalance Sensor: Abdulhalim Kilic1; Majid Jadidi1; Hakan Ozgur Ozer1; Fatma Nese Kok1; 1Istanbul Technical University
Dynamic adhesion behavior of human fetal osteoblastic bone cell lines was monitored by QCM-D. Cell experiments were repeated on hydrophobic, hydrophilic and phospholipid membrane coated gold sensors in cell culture medium with and without serum. Cell adhesion and later spreading was monitored for 17 h after an hour of seeding. In continuous flow, cells had spherical morphology, and adhered to the gold weakly through a few physical interactions yielding viscoelastic layer with high ΔD/Δf ratio. Slow decrease of -ΔD/Δf values were observed afterwards, showing increased stiffness probably because of cell spreading. On hydrophilic sensor, the amount of the attached cells (-Δf) was similar during the whole process, but ΔD and -ΔD/Δf ratio value was 2-fold lower indicating more flattened cells. There was no significant attached cell on lipid bilayer since it is a cell-resistant surface. Contribution of different molecules on cell behavior will be studied on different lipid bilayers.