Next Generation Biomaterials : Next Generation Biomaterials III
Sponsored by: ACerS Bioceramics Division, TMS Biomaterials Committee
Program Organizers: Roger Narayan, University of North Carolina; David Dausch, RTI International; Sanjiv Lalwani, Lynntech, Inc.
Tuesday 2:00 PM
November 3, 2020
Room: Virtual Meeting Room 12
Location: MS&T Virtual
Session Chair: Anna Bull, University of Tennessee Space Institute; Tzahi Cohen-Karni, Carnegie Mellon University; Ali Salifu, Worcester Polytechnic Institute
2:00 PM
Optimizing Bicontinuous Structure of Bijels-derived Polymer-hydrogel Hybrids for the Controlled Release of Different Cells: Haoran Sun1; Min Wang1; 1University of Hong Kong
In tissue engineering, controlled cell release requires suitable vehicles for cells. Bijels-derived bicontinuous structures have interconnected channels which provide desired space for cell encapsulation, proliferation and migration. Our research develops new methods for fabricating bijels and bijels-derived structures with suitable channel (pore) sizes. In this investigation, bijels-derived polymer-hydrogel hybrids with normal pore size (~30 um) and large pore size (~60 um) were produced, and human dermal fibroblasts (HDFs) and osteoblast precursor cell line (MC3T3 cells) were encapsulated in the crosslinked alginate hydrogel in respective hybrid. The hybrids were then cultured to study cell release. It was observed that live cells were locked in hybrid structures and were gradually released during culture. Both HDFs and MC3T3 cells exhibited good cell viability and proliferation after their release and were able to migrate through channels to the surface of hybrids and then culture plates. The two types of cells displayed different release behaviors.
2:20 PM Invited
Decellularized Lucky Bamboo (Dracaena sanderiana) Scaffolds for Bone Tissue Engineering: Ali Salifu1; Joshua Gershlak1; John Obayemi1; Vanessa Uzonwanne1; Glenn Gaudette1; Winston Soboyejo1; 1Worcester Polytechnic Institute
Many of the proposed alternative strategies for the repair of non-union bone defects rely on the development of synthetic scaffolds for bone regeneration that are limited by their structure and scale-up capabilities. With its well-organized, hierarchical and functionally graded structure, and its potential for large scale cultivation, lucky bamboo is presented as a cost-effective, scalable alternative for scaffold development. Hence, we developed decellularized lucky bamboo scaffolds functionalized with arginine-glycine-aspartate (RGD) peptides and characterized their physicochemical properties. The attachment, proliferation, and organization of human fetal osteoblast (hFOB) cells, and extracellular matrix (ECM) production and mineralization were demonstrated. The mechanical properties of the cell-laden decellularized scaffolds were also studied at different times and were shown to evolve at different stages of cell proliferation, ECM production, and mineralization. The data suggest that decellularized lucky bamboo scaffolds may provide biological material for use in bone repair in an environmentally and economically sustainable way.
2:40 PM Invited
Diamond-like Carbon Thin Films for an Improved Surgical Field of View: Anna Bull1; Jackson Mayfield1; Adam Evans1; Russell Leonard1; Jacqueline Johnson1; 1University of Tennessee Space Institute
The purpose of this research is to develop a coating for laparoscope lenses with the ability to overcome current fogging and contamination difficulties, allowing a clear field of view during surgery. Diamond-like carbon (DLC) coatings can be used to enhance the performance of the lenses due to their anti-fogging and anti-fouling characteristics. These properties are desirable, as they eliminate the need for intra-operative cleaning; the scratch-resistant nature of DLC provides additional benefits. The DLC films were produced by pulsed laser deposition, with dopants such as silicon and oxygen added to enhance their favorable properties. The films were characterized to determine water contact angle, surface energy, transparency, and scratch resistance, among other things. The biocompatibility of these films was analyzed through MTT assays and additional methods to ensure safe insertion into the human body. These thin films are an attractive method for achieving effective, long-lasting anti-fogging, anti-fouling coatings in medical applications.
3:00 PM
Mechanical Behavior of Resilin-mimicking Materials: Annaliza Perez-Torres1; Fuqian Yang1; 1University of Kentucky
Natural resilin, an elastomeric protein found in many insects and arthropods, has gained attention due to its unique properties, such as large strain, high resilience, efficient energy storage and exceptional cyclic lifetime at a micro/nanoscale. Due to these outstanding properties, it is of great importance to produce man-made materials to mimic the properties of natural resilin for potential applications in biodevices and systems. In this work, we synthesize resilin-mimicking hydrogels from Polydimethylsiloxane (PDMS), Polyethylene glycol (PEG), and Polyacrylamide (PAAm) and study the contact deformation of the hydrogels. The contact modulus and energy dissipation during the contact deformation are characterized. We use finite element analysis to simulate the contact deformation of the hydrogels and compare numerical results with experimental data.