Next Generation Biomaterials : Poster Session
Sponsored by: ACerS Bioceramics Division, TMS Biomaterials Committee
Program Organizers: Roger Narayan, University of North Carolina; David Dausch, RTI International; Sanjiv Lalwani, Lynntech, Inc.
Tuesday 9:00 AM
November 3, 2020
Room: Poster Hall
Location: MS&T Virtual
Session Chair: Roger Narayan, North Carolina State University; David Dausch, RTI International; Sanjiv Lalwani, Lynntech, Inc.
3D Printed Porous Tissue Engineering Scaffolds with the Self-folding Ability and Controlled Release of Growth Factor: Jiahui Lai1; Junzhi Li1; Min Wang1; 1University of Hong Kong
3D printing technologies enable the fabrication of porous scaffolds with good control over their architectures and properties. 3D printed scaffolds with shape morphing ability can reshape themselves after implantation to match the defect and anatomy of host tissues. Growth factors are often used in tissue engineering to accelerate tissue regeneration, and vascular endothelial growth factors (VEGF) enhances the regeneration of gastrointestinal tract and vasculature. In this study, an extrusion-based 3D printing system was used to construct porous scaffolds which have abilities of self-folding upon heating to the human body temperature and controlled release of VEGF. For 3D printed scaffolds, the self-folding ability was achieved by using poly(D, L-lactide-co-trimethylene carbonate) (PDLLA-TMC) which could change shape at a temperature greater than 37°C, while the controlled release of growth factor was achieved by using gelatin methacrylate (GelMA) as the functional layer to load VEGF. Different techniques were used to evaluate the printed scaffolds.
3D Printed Strontium-doped Alginate-collagen Scaffolds for Bone Tissue Engineering: Shams Khondkar1; Naomi Edwards1; Azhar Ilyas1; 1New York Institute of Technology
Despite all the advancements and technology, 5-10% of bone fractures are either nonunion or delayed. Treatment for critical size defects (CSDs) in bone often use bone grafts to act as a scaffold to help complete bony union healing. Biological scaffolds require bone extraction from the individual or an outside donor while synthetic grafts mostly suffer from poor degradation kinetics and decreased bioactivity. In this study, we propose a new bioink composed of alginate, collagen and doped with strontium-calcium polyphosphate (SCPP) to improve degradability and osteogenic/angiogenic properties of 3D printed scaffolds produced from this bioink. The characterization of the mechanical properties and degradation behavior of these scaffolds will be presented. We will also present results of cell-surface interactions via in vitro testing of these scaffolds for cell growth, proliferation and the mineralized tissue formation. The enhanced osteogenic effect of printing osteoblasts within these scaffolds will also be presented and discussed.
Biocompatible Ceramics Based on Hydrated Calcium Phosphates: Tatiana Safronova1; Gilyana Kazakova1; Otabek Toshev1; Andrey Kiselev1; Tatyana Shatalova1; Irina Selezneva2; Vladimir Zaitsev3; 1Lomonosov Moscow State University; 2Institute of theoretical and experimental biophysics of RAS; 3Priorov National Medical Research Center of Traumatology and Orthopedics
Materials based on calcium pyrophosphate Са2Р2О7 with a molar ratio Ca/P=1 and calcium polyphosphate Са(РО3)2 (in an amount up to 50%), having a molar ratio Ca/P=0.5 meet the requirements that necessary for application in regenerative medicine. Calcium phosphates with a molar ratio Ca/P=1 (Са2Р2О7.хН2О, СаНРО4.2Н2О, СаНРО4) synthesized from solutions, in pastes, or in conditions of mechanical activation were used as precursors of calcium pyrophosphate Са2Р2О7. Calcium phosphates with a molar ratio Ca/P=0.5 (Са(РО3)2.хН2О, Са(Н2РО4).Н2О, Са(NH4)2P2O7, СаH2P2O7) were used as precursors of calcium polyphosphate Са(РО3)2. Powder mixture comprising precursors of the calcium pyrophosphate and polyphosphate phases at a given molar ratio in the range 0.5<Ca/P<1 were used for production of ceramic materials in the system Са2Р2О7-Са(РО3)2. The target phases are formed as a result of thermal conversion and as a result of heterophase interaction in the investigated mixtures. The work was supported by RFBR, projects # 18-29-11079, # 20-03-00550.
Powder Mixtures of Calcium Hydroxyapatite and Potassium Hydrosulfate for Producing Biocompatble Ceramics: Tatiana Safronova1; Gilyana Kazakova1; Marat Akhmedov2; Tatyana Shatalova1; Snezhana Tikhonova1; 1Lomonosov Moscow State University; 2A.N. Kosygin State University of Russia (Technology. Design. Art)
Powder mixtures of hydroxyapatite Са10(РО4)6(ОН)2 and potassium hydrosulfate КНSO4 were investigated to confirm possibility of creating ceramic composites with biocompatible and bioresorbable phases of tricalcium phosphate Са3(РО4)2, potassium rhenanite KCaPO4, potassium-substituted tricalcium phosphate Ca10K(PO4)7 and calcium sulfate anhydrite СаSO4. Powder mixtures of calcium hydroxyapatite Са10(РО4)6(ОН)2 and potassium hydrosulfate КНSO4 were homogenized in acetone medium in planetary mill. Powder mixtures were made with molar ratio of КНSO4/Са10(РО4)6(ОН)2 established as 2/1, 4/1 and 6/1. In fact, the following phases were found in samples after firing in interval of 700-900oC: potassium calcium phosphate Ca10K(PO4)7, potassium calcium double sulfate K2Ca2(SO4)3 and potassium sulfate K2SO4. The obtained data indicate the need to adjust the composition of the staring powder mixtures for creating of ceramic composites in the K2O-CaO-SO3-P2O5 system with adequate for the intended use in regenerative medicine phase composition and solubility.Acknowledgements The financial support of RFBR project # 20-03-00550.